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Pulsed-field gel electrophoresis genotyping of Salmonella gallinarum and comparison with random amplified polymorphic DNA

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Authors
Seo, Yeon-Soo; Lee, Seung-Hoon; Shin, Eun-Kyung; Kim, Sun-Joong; Jung, Rose; Hahn, Tae-Wook
Issue Date
2006-04-11
Publisher
Elsevier
Citation
Vet. Microbiol. 115, 349–357
Keywords
Pulsed-field gel electrophoresis (PFGE)RAPDSalmonella gallinarumGenotyping
Abstract
Salmonella gallinarum is gram-negative bacteria that cause fowl typhoid (FT) in chickens. Since the first outbreak of FT
reported in 1992 in Korea, it has widely spread throughout the country. Today, FT is one of the most devastating diseases of
poultry. The aim of the present study was to ascertain a genetic relationship among S. gallinarum isolates collected from
different regions of Korea over a 10-year period. We examined a total of 38 isolates of S. gallinarum obtained in 29 regions of
Korea from 1992 to 2001 including the 9R vaccine strain and the standard strain of S. gallinarum (ATCC 9184). The PFGE
profiles produced 12 different patterns with the XbaI-digestion and 11 different patterns with the SpeI-digestion. The RAPD
using URP-6 primers showed eight different genotypes with the same Salmonella isolates. The PFGE patterns of the 9R vaccine
strain and ATCC 9184 of S. gallinarum were different from the identical type A, the most common genotype among field isolates
in our study. In conclusion, a low genetic heterogeneity was observed among Korean S. gallinarum isolates. In addition, PFGE
appeared to be a more accurate and reproducible method for genotyping of S. gallinarum isolates than RAPD.
ISSN
0378-1135
Language
English
URI
http://hdl.handle.net/10371/6542
DOI
https://doi.org/10.1016/j.vetmic.2006.02.019
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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