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One-Step Immunochromatography Assay Kit for Detecting Antibodies to Canine Parvovirus

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Authors
Oh, Jin-Sik; Ha, Gun-Woo; Cho, Young-Shik; Kim, Min-Jae; An, Dong-Jun; Hwang, Kyu-Kye; Lim, Yoon-Kyu; Park, BongKyun; Kang, BoKyu; Song, Dae-Sub
Issue Date
2006
Publisher
American Society for Microbiology
Citation
Clin. Vaccine Immunol. 13:520-524
Abstract
This study was performed to determine the feasibility of using whole serum to detect antibodies to canine
parvovirus (CPV) under nonlaboratory conditions and to evaluate the performance characteristics of an
immunochromatography assay kit. Precise detection of levels of antibody against CPV in puppies can be used
to determine a vaccination schedule, because maternal antibodies frequently result in the failure of protective
vaccination, and can also be used to determine the antibody levels of infected puppies. Several methods for the
titration of CPV antibodies have been reported, including the hemagglutination inhibition (HI) assay, which
is considered the “gold standard.” These methods, however, require intricate and time-consuming procedures.
In this study, a total of 386 serum specimens were tested. Compared to the HI assay, the rapid assay had a
97.1% sensitivity and a 76.6% specificity (with a cutoff HI titer of 1:80). This single-step assay could be
performed rapidly and easily without special equipment. The kit provides a reliable method for detection of
anti-CPV antibody where laboratory support and personnel are limited.
ISSN
1071-412X
Language
English
URI
http://hdl.handle.net/10371/6546
DOI
https://doi.org/10.1128/CVI.13.4.520-524.2006
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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