동종 및 이종 피부조직의 반복주사에 의한 실험적 피부염에 관한 연구

Abstract

An experimental study on dermatopathologic changes in albino rats induced by repeated injections of homologous and heterologous dermal tissue with Freund's complete adjuvant is carried out, as an attempt to produce immonologically related chronic dermatitis and to clarify the nature and significance of this type of lesion in morphological viewpoint. Followings are the results: 1. A reliable pathologic alterations of skin is only observed in the group of animals that were injected with homogenized rat dermal suspension with adjuvant. 2. Major histopatholgic changes of epidermis present hyperkeratosis, parakeratosis, keratotic pluggings, atrophy, and focal fraying and disruption of the basement membrane and a rare liquefaction. In the dermis are moderate degree of sclerosis, agg regates of mixed inflammatory ceIls, especiatly around small vascuIatures, subepiderlmal edema and focal vasculitis. Interstitial myositis is an another association. These forms of changes are able to divide the lesions in two groups: ceIlular exudative and dermal sclerotic. 3. The ceIlular exudative from is of a chronic nonspecific dermatitis similar to that of human disorder, and the dermal sclerotic variety resembles scleroderma and/or dermatomysitis. 4. Adjuvant granulom-as and their necroses are more extensive in the same group of animals, and often suggestive Arthus phenomenon is manifest near the site of injection. 5. Systemic tissue alteralion is not apparent in relation to immunological cross reaction with dermal collagen, except for the presence of adjuvant reaction. From the above morphologic features, the experimental dermatitis presently described exhibits many similarities to those of chronic dermatitis of unknown etiology and/or to scleroderma-dermatomyositis line, and it is suggested that an immunological process may anticipate in one of the pathogenic pathway of human chronic dermatitis.