A Study on Some Problems in PFAAB-PAS-OG Staining Method

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Baik, Sang Ho; Chang, Ka Young; Lee, Kwang Ho
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서울대학교 의과대학
Seoul J Med 1971;12(3):164-165
The most critical and imporant step in
PFAAB-PAS-OG staining method is the oxidation
in the strong acidic solution. Although
alternative oxidants such as peracetic acids,
periodic acids and acid perrnanganate could be
employed instead of performic acid, many
investigators prefer the latter because of its
better staining results. Since performic acid is
made of formic acid, cone. sulfuric acid
and hydrogen peroxide, it makes the tissue
swollen unfortunately and finally let the
sections come off the slide.
Celloidin, which have been used as an
embedding media, have good physical and
chemical character in many respects. One of
them is the fact that celloidin passes staining
dyes and other chemical solutions easily without
disturbing the effect of the solutions.
In our experiments, some mucoid cells were
oxidized across the celloidin film and appeared
as alcian blue stained blue granules, while the
remains which were intact to the oxidants and which contains either no cystine or an insufficient
amount of cystine to give a positive alcian
blue reaction appeared as PAS-positive magenta
granules. With the alcian blue stain, celloidin
has been colored as deep blue and the color
was remained even after 24 hours rinsing in the
running tap water, or in the acidic solutions.
To overcome the difficulties of oxidation step
in PFAAB-PAS-OG reaction, tissue sections
were coated with celloidin film before immersing
in acidic solution and obtained good staining
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 12 No.3 (1971)
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