인삼 Saponin Fraction이 흰쥐 대뇌피질 Mitochondrial ATPase에 미치는 영향

Abstract

Mitochondria catalyze a group of four reactions which are believed to represent individual steps or partial reacitons in the A'I'Pr-forming process. These reactions take place in the absence of net flow of electrons down the respiratory chain. The first of these reactions is the ATPase activity of mitochondria, which is normaIly very low but which is greatly stimulated by DNP, so-called "activation of Latent ATPase activity". In an attempt to evaluate and compare the effects on ATP ase activity of the Ginseng saponin, DNP, ouabain and deoxycholate, the experiments were perfermed with mitochondria obtained from rat brain cortex. The results are summerized as follow; 1. Mitochondrial ATPase activity was increased by Ginseng saponin especially when saponin was preincubated with mitochondria. 2. ATPase activity was increased by deoxycholate but of which effect did not influence on the action of DNP on ATPase activity. 3. Ginseng saponin significantty elevated the altered ATPase activity by DNP. 4. Ginseng saponin did not influence the ATPase activity elevated by deoxycholate. 5. Ginseng saponin significanty elevated A'I'Pase activity which was depressed by ouabain. 6. It is suggested that Ginseng saponin enhanced mitochondrial ATPase activity through the direct action upon the ATPase system rather than the alteration of mitochondrial membrane structure.