첨가 동종 타액선조직의 반복주사에 의한 백서 타액선염에 관한 실험적 연구

Abstract

A study of experimental sialadenitis was carried out by means of repeated injections of homologous salivary gland suspension with Freund's complete adjuvant in rats, as an attempt to produce immunerelated sialadenitis and to clarify the nature of this type of lesion, in relation to Sjogren's syndrome and certain chronic non-specific sialadenitis. The results were as follows: 1. A significant inflammatory change of salivary gland was observed in the group of animals for which homologous salivary gland suspension with Freund's complete adjuvant was injected. Significant sialadenitis was evident in 9 mucous glands and 14 serous glands of 15 animals, respectively. The major histologic alterations included atrophy and degeneration of acini with occasional intraluminal inspissation of secretory substance, duct ectasia with or without stagnation of inspissated secretion, and focal or diffuse infiltration of lymphocytes, pseude-eosinophils, plasma cells and histiocytes with a rare lymphoid follicle formation, the latter assuming roughly correlated with severity of the formers. 2. Less apparent inflammatory change was demonstrated in the animal groups for which salivary gland suspension was given alone than those added with Freund's complete adjuvant. 3. The other animal groups exhibited no significant sialadenitis or evidence of cross-reaction in other organs. 4. Two types of changes were observed in groups of animals received either adjuvant alone or with tissue suspension, the one is non-caseating granuloma formation in the injected site of skin, lung and liver; the another is marked hyperplasia of reticuloendothelial cells of spleen, lungs, and liver with enhanced infiltration of plasma cells and lymphocytes. From the above histopathologic findings, the experimental sialadenitis presently described shows many similarity to those of Sjogren's syndrome and/or certain chronic non-specific sialadenitis, and it is assumed that an immunological process may be considered as one of the pathogenetic pathways of Sjogren's syndrome or certain chronic sialadenitis.