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Altered Electrophoretic Mobility of the Human Erythrocytic Glucose-6-phosphate Dehydrogenase as a Function of their in vivo Ages

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Authors
Lee, Choon-Shick; Kimm, Sung-Wun
Issue Date
1971-12
Publisher
서울대학교 의과대학
Citation
Seoul J Med 1971;12(4):293-302
Abstract
Human red cells were separated, by centrifugation
by virtue of their density gradient
due to lipid contents, into the younger and
older cells.
Lipid analysis confirmed that the older red
cells have lower content than their younger
conterparts.
Crude G6PD's derived from each younger
and older red cells were subjected to cellulose
acetate electrophoresis, and their activities
were assayed spectrophotometrically. The
activity declined apparently as aging takes
place, with little or no changes in their glutathione
contents.
Formazan bands on electropherograms of
both G6PD's derived from both cells clearly
disclosed the fact that the G6PD from the
older cell migrates roughly 1.2 times faster
toward anode than the enzyme from the
younger cell. The alteration of the G6PD
mobility as well as its activity was more
accentuated with additon of 2-mercaptoethanol
and cysteine.
Language
English
URI
http://hdl.handle.net/10371/6600
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Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 12 No.4 (1971)
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