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환경온도 및 배지성분의 차이가 거식세포 식균작용에 미치는 영향
Influences of temperature and media on the phagocytic activities of rabbit macrophage

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Authors
박원철
Issue Date
1974-03
Publisher
서울대학교 의과대학
Citation
Seoul J Med 1974;15(1):18-26
Abstract
Bacterial suspensions of Staph. aureus and Es,
coli cultivated in Brain-Heart Infusion Broth were
mixed with macrophages collected from rabbit peritoneal
cavities in the Cell-Maintenance Fluid(C. M.
F.), physiological saline solution, Ringer sol. and
normal rabbit serum, so as to make bacterial number
being about 10'/cc and macrophage number being
10'/cc. The macrophage-bacterial suspensions were maintained
at room temperature(24'C-26'C) and 37'C,
then the percentages of macrophages with intracellular
organisms and microbes located intracellularly
and also the average number of intracellular organisms
were investigated after 15 and 30 minutes.
Results were summerized as follows:
1) In the Staph. aureus-rnacrophage suspension;
The percentages of macrophages with intracellular
organisms in eMF, saline, Ringer sol. and normal
rabbit serum were 58%,34. 5%, 25% and 43.5% after
15 minutes, and 87.5%, 49%, 26% and 83.5% after
30 minutes, respectively at 37'C.
At room temperature(24'C-26'C), the percentages
of macrophages with intracellular organisms in CMF,
saline, Ringer sol. and normal rabbit serum were
23%, 19.5%, 20.5% and 47.5% after 15 minutes,
and 33%, 27.5%, 28% and 67% after 30 minutes,
respectively.
The percentages of intracellular organisms in
CMF, saline, Ringer sol. and normal rabbit serum
were 80%, 24%, 26. 5% and 58.5% after 15 minutes,
and 92. 5%, 30%, 31. 5% and 80% after 30 minutes,
respectively at 37'C.
At room temperature, the percentages of intracellular
organisms in eMF, saline, Ringer sol. and
normal rabbit serum were 71%, 23%, 28.5% and
71. 5% after 15 minutes, and 69%, 41. 5%, 40% and
81% after 30 minutes, respectively.
Average number of intracellular organisms in
CMF, saline, Ringer sol. and normal rabbit serum
were 5, 3. 5, 3 and 3 after 15 minutes, and 11, 6. 5,
5 and 8.5 after 30 minutes, respectively at 37'C.
At room temperature, average number of intracellular
organisms in eMF, saline, Ringer sol. and
normal rabbit serum were 3, 2.5, 2 and 4 after 15
minutes, and 5, 3, 3 and 6. 5 after 30 minutes,
respectively.
2) In the Es, coli-Macrophages suspension;
The percentages of macrophages with intracellular
organisms in eMF, saline, Ringer so]. and normal
rabbit serum were 37.5%, 34%, 12.5% and 49.5%
after 15 minutes, and 73%, 76.5%, 13.5% and 76.5
% after 30 minutes, respectively at 37'C.
A t room temperature. the percentages of macrophages
with intracellular organisms in CMF, saline,
Ringer sol. and normal rabbit serum were 36%, 26
%, 12% and 36% after 15 minutes, and 32.5%, 17
%, 14% and 65% after 30 minutes, respectively. The percentages of intracellular organisms in
CMF, saline, Ringer sol. and normal rabbit serum
were 79. 5%, 72.5%, 43. 5% and 78. 5% after 15
minutes, and 92.5%, 77%, 48.5% and 82% after 30
minutes, respectively at 37'C.
At room temperature, the percentages of intracellular
organisms in eMF, saline, Ringer sol. and
normal rabbit serum were 84.5%, 61%, 45% and
70% after 15 minutes, and 83.5%, 67%, 34.5% and
84% after 30 minutes, respectively.
Average number of intracellular organisms in
CMF, saline, Ringer sol. and normal rabbit serum
were 2, 2, 1.5 and 2. 5 after 15 minutes, and 3, 3,
1.5 and 3.5 after 30 minutes, respectively at 37'C.
At room temperature, average number of intracellular
organisms in eMF, saline, Ringer sol. and
normal rabbit serum were 1. 5, 2, 1. 5 and 2. 5 after
15 minutes. and L 1.5, 1.5 and 2. 5 after 30 minutes,
respectively.
Language
Korean
URI
http://hdl.handle.net/10371/6603
Files in This Item:
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 15 No.1 (1974)
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