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환경온도 및 배지성분의 차이가 거식세포 식균작용에 미치는 영향 : Influences of temperature and media on the phagocytic activities of rabbit macrophage
DC Field | Value | Language |
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dc.contributor.author | 박원철 | - |
dc.date.accessioned | 2009-08-07T15:19:35Z | - |
dc.date.available | 2009-08-07T15:19:35Z | - |
dc.date.issued | 1974-03 | - |
dc.identifier.citation | Seoul J Med, Vol.15 No.1, pp. 18-26 | - |
dc.identifier.issn | 0582-6802 | - |
dc.identifier.uri | https://hdl.handle.net/10371/6603 | - |
dc.description.abstract | Bacterial suspensions of Staph. aureus and Es,
coli cultivated in Brain-Heart Infusion Broth were mixed with macrophages collected from rabbit peritoneal cavities in the Cell-Maintenance Fluid(C. M. F.), physiological saline solution, Ringer sol. and normal rabbit serum, so as to make bacterial number being about 10'/cc and macrophage number being 10'/cc. The macrophage-bacterial suspensions were maintained at room temperature(24'C-26'C) and 37'C, then the percentages of macrophages with intracellular organisms and microbes located intracellularly and also the average number of intracellular organisms were investigated after 15 and 30 minutes. Results were summerized as follows: 1) In the Staph. aureus-rnacrophage suspension; The percentages of macrophages with intracellular organisms in eMF, saline, Ringer sol. and normal rabbit serum were 58%,34. 5%, 25% and 43.5% after 15 minutes, and 87.5%, 49%, 26% and 83.5% after 30 minutes, respectively at 37'C. At room temperature(24'C-26'C), the percentages of macrophages with intracellular organisms in CMF, saline, Ringer sol. and normal rabbit serum were 23%, 19.5%, 20.5% and 47.5% after 15 minutes, and 33%, 27.5%, 28% and 67% after 30 minutes, respectively. The percentages of intracellular organisms in CMF, saline, Ringer sol. and normal rabbit serum were 80%, 24%, 26. 5% and 58.5% after 15 minutes, and 92. 5%, 30%, 31. 5% and 80% after 30 minutes, respectively at 37'C. At room temperature, the percentages of intracellular organisms in eMF, saline, Ringer sol. and normal rabbit serum were 71%, 23%, 28.5% and 71. 5% after 15 minutes, and 69%, 41. 5%, 40% and 81% after 30 minutes, respectively. Average number of intracellular organisms in CMF, saline, Ringer sol. and normal rabbit serum were 5, 3. 5, 3 and 3 after 15 minutes, and 11, 6. 5, 5 and 8.5 after 30 minutes, respectively at 37'C. At room temperature, average number of intracellular organisms in eMF, saline, Ringer sol. and normal rabbit serum were 3, 2.5, 2 and 4 after 15 minutes, and 5, 3, 3 and 6. 5 after 30 minutes, respectively. 2) In the Es, coli-Macrophages suspension; The percentages of macrophages with intracellular organisms in eMF, saline, Ringer so]. and normal rabbit serum were 37.5%, 34%, 12.5% and 49.5% after 15 minutes, and 73%, 76.5%, 13.5% and 76.5 % after 30 minutes, respectively at 37'C. A t room temperature. the percentages of macrophages with intracellular organisms in CMF, saline, Ringer sol. and normal rabbit serum were 36%, 26 %, 12% and 36% after 15 minutes, and 32.5%, 17 %, 14% and 65% after 30 minutes, respectively. The percentages of intracellular organisms in CMF, saline, Ringer sol. and normal rabbit serum were 79. 5%, 72.5%, 43. 5% and 78. 5% after 15 minutes, and 92.5%, 77%, 48.5% and 82% after 30 minutes, respectively at 37'C. At room temperature, the percentages of intracellular organisms in eMF, saline, Ringer sol. and normal rabbit serum were 84.5%, 61%, 45% and 70% after 15 minutes, and 83.5%, 67%, 34.5% and 84% after 30 minutes, respectively. Average number of intracellular organisms in CMF, saline, Ringer sol. and normal rabbit serum were 2, 2, 1.5 and 2. 5 after 15 minutes, and 3, 3, 1.5 and 3.5 after 30 minutes, respectively at 37'C. At room temperature, average number of intracellular organisms in eMF, saline, Ringer sol. and normal rabbit serum were 1. 5, 2, 1. 5 and 2. 5 after 15 minutes. and L 1.5, 1.5 and 2. 5 after 30 minutes, respectively. | - |
dc.language.iso | ko | - |
dc.publisher | 서울대학교 의과대학 | - |
dc.title | 환경온도 및 배지성분의 차이가 거식세포 식균작용에 미치는 영향 | - |
dc.title.alternative | Influences of temperature and media on the phagocytic activities of rabbit macrophage | - |
dc.type | SNU Journal | - |
dc.contributor.AlternativeAuthor | Park, Won-Chul | - |
dc.citation.journaltitle | 서울 의대 잡지 | - |
dc.citation.journaltitle | 서울 의대 학술지 | - |
dc.citation.journaltitle | Seoul Journal of Medicine | - |
dc.citation.endpage | 26 | - |
dc.citation.number | 1 | - |
dc.citation.pages | 18-26 | - |
dc.citation.startpage | 18 | - |
dc.citation.volume | 15 | - |
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