내분비 변조에 다른 흰쥐 뇌하수체 난포 자극호르몬 세포의 변화

Abstract

Follicle stimulating hormone cells and its granules of rat adenohypophysis under various status of hormonal alteration have been studied. To induce the alteration of endocrine condition. gonadectomy or long term administration of estradiol has been carried out before sacrifice. Pituitary glands from the rats of estrous cycle and in gestation were also studied. Tissue sections of the glands were reacted with specific rabbit antiserum to the ovine FSH and followed by anti-rabbit gamma globulin labeled with horseradish peroxidase. The enzyme wail localized histochemically with diaminobenzidine. Number of FSH cells was reduced after long term administration of estradiol while castration. estrous cycle and gestation did not affect significantly. The most intense reactions of granules were observed in the pituitary glands of castrated group and the least intense reactions from the rats in estrus. Rats from thirty days after gonadectomy and rats in proestrus revealed well granulated FSH cells while the rats in estrus revealed poor granulation. Only the castrated rats showed increase of hypophyseal weight. No FSH granules could be observed in the adenohypophysis of new born rats.