Publications
Detailed Information
개의 대뇌후두엽 조직의 Guanine Aminohydrolase에 관한 연구 : A Study on the Guanine Arninohydrolase in the Tissue of Occipital Lobe of Dog Brain
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 1974-09
- Publisher
- 서울대학교 의과대학
- Citation
- Seoul J Med, Vol.15 No.3, pp. 175-183
- Abstract
- Crude preparation of the guanine aminohydrolase
was obtained from the occipital lobe tissue of dog brain by means of salting-out with ammonium sulfate,
and the enzymatic properties were observed
with the following conclusions.
(l) The KM value of the crude GDA toward the
substrate. guanine, was 0.26mM.
(2) The GDA was highly heat-stable. and inactivated
by urea and guanidine-Hfll, the magnitude of
the inhibition by the latter being more prominant
than the former.
(3) The GDA had a single and broad spectrum of
its pH-versus activity profile, ranging from pH 7.0
to 9.0.
(4) Among the inorganic ions examined, Cat,
Cu++, Mrrr", Fet", Fe+++, Pb!', Co":", CN- displayed
no effect on the GDA activity, whereas Hgtt showed
100% and Mgtt about 10% inactivation of the
enzyme.
(5) There was no evidence of the presence of the
natural inhibitor of the present GDA in the mitochondrial
fraction of the tissue of the occipital lobe
of dog brain, and of product inhibition by xanthine
and hypoxanthine as well.
(6) There was an indication of possible allosteric
property in the present preparation of GDA with
GTP as its positive modifier against the alleged
cooperative phenomenon.
- ISSN
- 0582-6802
- Language
- Korean
- Files in This Item:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.