백일해균의 가용성 항원에 관한 연구

Abstract

It was well established that the inoculation of pertussis vaccine was capable of reducing the incidence of whooping cough. However, complete elimination of toxicity of pertussis vaccine was not succeeded yet. Thus this experiment was carried out to reduce the toxicity of pertussis vaccine. This paper described the conditions under which sodium desoxycholate may be used to liberate the the soluble protective antigen in nontoxic form and the potency of protective antigen liberated from both 48 hrs. and 72 hrs. pertussis cultures. Results were as follows: 1. The liberation of soluble protective antigen from pertussis cells in non-toxic form was possible using both optimal concentration (0.5%) and pH(8.5) of sodium desoxycholate solution. 2. The protective antigen liberated from 48hrs. pertussis culture at 0.5% sodium desoxycholate solution exhibited a greater degree of potency than that liberated from 72hrs. cultures.