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N-acetylcysteine stimulates osteoblastic differentiation of mouse calvarial cells

Cited 45 time in Web of Science Cited 44 time in Scopus
Authors
Ryoo, Hyun-Mo; Jun, Ji Hae; Lee, Sun-Hwan; Kwak, Han Bok; Lee, Zang Hee; Seo, Sang-Beum; Woo, Kyung Mi; Kim, Gwan-Shik; Baek, Jeong-Hwa
Issue Date
2008
Publisher
Wiley-Blackwell
Citation
Journal of Cellular Biochemistry 103:1246-1255
Keywords
N-acetylcysteineosteoblast differentiationglutathioneinhibition of RhoA activity
Abstract
Estrogen deficiency causes osteoporosis via increased generation of reactive oxygen species (ROS), and thus, antioxidants may prove to be the effective therapeutic candidates. We examined the effects of the antioxidant N-acetylcysteine (NAC) on osteoblastic differentiation in mouse calvarial cells. NAC (10-30 mM) enhanced alkaline phosphatase activity, mRNA expression of osteoblast differentiation-associated genes and mineralized nodule formation. It also increased expression of bone morphogenetic proteins-2, -4, and -7. The osteogenic activity of NAC was partially reduced by inhibition of glutathione synthesis. Since caffeic acid phenethyl ester did not stimulate osteoblast differentiation, it is unlikely that ROS scavenging activity of NAC is sufficient for osteogenic activity. We observed that NAC suppressed small GTPase RhoA activity and activation of RhoA by Pasteurella multocida toxin suppressed the osteogenic activity of NAC. These results suggest that NAC might exert its osteogenic activity via increased glutathione synthesis and inhibition of RhoA activation.
ISSN
0730-2312
Language
English
URI
http://hdl.handle.net/10371/66630
DOI
https://doi.org/10.1002/jcb.21508
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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