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Decreased expression of alpha3 and beta1 integrin subunits is responsible for differentiation-associated changes in cells behavior in terminally differentiated human oral keratinocytes.
DC Field | Value | Language |
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dc.contributor.author | Oh, Ju-Eun | - |
dc.contributor.author | Park, Kyung-Hee | - |
dc.contributor.author | Noh, Hyung Kil | - |
dc.contributor.author | Kim, Jin-Man | - |
dc.contributor.author | Chung, Chong-Pyoung | - |
dc.contributor.author | Min, Byung-Moo | - |
dc.date.accessioned | 2010-05-17T23:55:24Z | - |
dc.date.available | 2010-05-17T23:55:24Z | - |
dc.date.issued | 2002-07 | - |
dc.identifier.citation | Cell Commun Adhes 9(4):173-187 | en |
dc.identifier.issn | 1541-9061 | - |
dc.identifier.uri | https://hdl.handle.net/10371/66634 | - |
dc.description.abstract | Primary normal human oral keratinocytes (NHOKs) terminally differentiate in serial subculture.
To investigate whether this subculture-induced differentiation of NHOKs affects integrin expression and cell-matrix interaction, we studied the expression levels of integrin subunits and cellular response to the extracellular matrix (ECM) proteins in NHOKs at different population doublings. The phosphorylation statuses of focal adhesion kinase (FAK), extracellular signal regulated kinase (ERK), p38, and c-Jun amino-terminal kinase (JNK) were also determined in NHOK cells cultured on ECM proteins, to evaluate the functions of integrins with respect to cellular responses to ECM proteins. The expression levels of ®3 and ¯1 integrin subunits progressively decreased in NHOKs undergoing terminal differentiation. The ability of NHOKs to spread upon laminin and type I collagen significantly decreased in terminally differentiated oral keratinocytes. Keratinocyte migration was significantly increased on type I collagen for terminally differentiated NHOKs. Similar results were seen following preincubation of rapidly proliferating NHOKs with function-blocking antibodies to ®3 or ¯1 integrin subunit. In contrast, fibronectin had no effect on cellular responses in NHOKs, which were almost negligible in the expression levels of ®5 integrin subunits. The extent of FAK phosphorylation in terminally differentiated NHOKs was notably lower than that of rapidly proliferating cells, but was enhanced in terminally differentiated cells that were cultured on type I collagen. Our results indicate that decreased expression of ®3 and ¯1 integrin subunits is responsible for differentiation-associated changes in cells behavior in terminally differentiated oral keratinocytes. Our data also show that the abrogation of the ®5¯1 integrin function caused by omitting ®5 subunit is linked to the loss of a cell-fibronectin interaction in human oral keratinocytes. | en |
dc.description.sponsorship | This study was supported by the Korean Science
and Engineering Foundation (KOSEF) through the Intellectual Biointerface Engineering Center (IBEC) at the Seoul National University. | en |
dc.language.iso | en | en |
dc.publisher | Taylor & Francis | en |
dc.subject | Cells behavior | en |
dc.subject | differentiated human oral keratinocytes | en |
dc.subject | ECM proteins | en |
dc.subject | integrins | en |
dc.subject | signaling molecules | en |
dc.title | Decreased expression of alpha3 and beta1 integrin subunits is responsible for differentiation-associated changes in cells behavior in terminally differentiated human oral keratinocytes. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 오주은 | - |
dc.contributor.AlternativeAuthor | 박경희 | - |
dc.contributor.AlternativeAuthor | 노형길 | - |
dc.contributor.AlternativeAuthor | 김진만 | - |
dc.contributor.AlternativeAuthor | 정종평 | - |
dc.contributor.AlternativeAuthor | 민병무 | - |
dc.identifier.doi | 10.1080/154190602901116410 | - |
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