저장 혈액중에 있는 임파구의 성상 변화에 관한 연구

Abstract

Changes in the ability of lymphocytes in stored bank blood to form E-rosettes and to respond to PHA were studied together with change of other blood cell components at intervals during two to three weeks of storage at 4T in ACD-B and CPD plastic bags. The results were as follows. 1. There was no significant change in red blood cell count, hemoglobin and hematocrit during 21 days of storage. 2. Leucocyte count decreased to 90% of the initial value after 1 week and then there was no significan t decrease up to 3 weeks of storage. Viability of lymphocytes was maintained up to two weeks at 9096 level and that of neutrophil declined rapidly to 1/3 of the initial value after 1 week's storage. 3. Platelet count decreased rather slowly and 60% of the initial value "vas present after 3 weeks' storage. 4. E-rosette formation of lymphocytes was relatively well maintained during the first 3 days and decreased to 2/3 on day 4, and to less than 1/2 after 10-14 days' stroage. 5. Spontaneous and PHA stimulated lymphocyte blastogenesis decreased progressively during storage. but was not completely abolished after 14-17 days' storage.