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Chloroplast Gene Marker를 利用한 현사시나무 選拔 clone間 Restriction Fragment Length Polymorphism 分析
Restriction Length Polymorphism Analysis of Populus alba x P. hlandulosa F1 clones using Chloroplast Gene Markers

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Authors
洪庚洛; 金用律; 玄正悟
Issue Date
1990
Publisher
The Arboretum, Seoul National University
Citation
Bulletin of the Seoul National University Arboretum, no.10, pp.22-32
Abstract
The total DNA samples, isolated from 4 clones of P. alba x P. glandulosa were digested with 6restriction enzymes and hybridized with P-labeled gene probe from pCB16, pCB19 and pCB35 containing 2-4kb of Chloroplast DNA. When DNA samples were digested with ClaI, EcoRI and
BamHI and hybridized with the probes from pCB16 and pCB29, we found the same band pattern clone
1 and 3 and also between clone 2 and 4. However, restriction site variation was found between clone 1
and 2 and also between clone 3 and 4. This result is in good agreement with the fact that the pollen
parent is common for all 4 clones while clone 1 and 3 share the same seed parent and clone 2 and 4
share another same seed parent. This indicate that the inheritance of chloroplastal DNA in Populus
may be maternal and that the 'RFLP' method used in our study can be useful for identification of
aspen clones.
ISSN
1226-7600
Language
Korean
URI
http://hdl.handle.net/10371/67412
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College of Agriculture and Life Sciences (농업생명과학대학)The Arboretum, Seoul National University (수목원)Bulletin of the arboretum Seoul National University (수목원 연구보고)Bulletin of the arboretum Seoul National University no.10 (1990)
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