Amplification of VNTR Locus D1S80 in DNA Recovered from Vaginal Sperm

Abstract

Sperm DNA was isolated from vaginal fluid and/or endocervical mucoid plugs at various postcoital intervals. Samples were obtained from 25 volunteer couples. DNA was also prepared from the blood and semen of the couples. The DNA was amplified by polymerase chain reaction (peR) with D1S80 primers. The Fragment length polymorphism of the amplified products (AMP-FLP) was analysed by acrylamide gel electrophoresis followed by ethidium bromide staining. The AMP-FLP of the vaginal fluid was identical to that of the blood of the male partner within 10 hours after coitus. Thereafter, the male DNA recovery rate reduced as the collection interval extended. After 76 hours no male DNA profile was detected in the vaginal sample. Therefore, AMP-FLP analysis of the D1S80 can be used to exclude to determine the probable identity of an assailant in rape cases.