Immunohistochemical Study on Changes of the T- and B-Cell Population in Uterus Draining Lymph Nodes of Mouse during Preqnancy
자궁유출림프절에서 T 및 B 세포계의 임신기간에 따른 변화에 관한 면역조직화학적 연구

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Lee, Wang Jae; Hwang, Young Sil; Hwang, Douk Ho; Lee, Kwang Ho; Chang, Ka Young
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Seoul National University College of Medicine
Seoul J Med 1991;32(4):177-189
Histochemical and immunohistochemical stainingPregnant miceUterus draining lymph nodesActivated B cellsIgM- and IgG positive cellsL3T4- and Lyt-2 positive cell
The following experiment was performed to investigate changes of immune
status in the uterus draining lymph nodes of pregnant ICR mice. The experimental
animals were divided by duration of pregnancy as follows; Group I, virgin control; Group
II, pregnancy 2nd day; Group III, pregnancy 5th day; Group IV, pregnancy 8th day;
Group V, pregnancy 14th day; Group VI, pregnancy 19th day. The objective of this
study was to focus on the changes in both B and T-cell systems. To note changes
in the B-cell system, the nunber and distribution pattern of activated B cells were observed,
the number of IgM and IgG positive plasma cells was counted" and the distribution
pattern of surface IgM and IgG positive cells was observed. For changes in the T cell
system, the distribution pattern of L3T4 positive and Lyt-2 positive cells was observed.
Histochemical staining for activated B cells by means of membrane alkaline phosphatase
activity (mAP) and immunohistochemical staining, such as avidin-biotin-peroxidase
complex (ABC) and alkaline phosphatase-anti-alkaline phosphatase (APAAP) techniques
for T cell and other B-cell systems were carried out. L3T4 positive cells in the deep
cortex and IgM- and IgG-positive plasma cells in the medulla were significantly increased
near the term. Lyt-2 positive cells were increased in the medullary cord or corticomedullary
junction on the 5th day of pregnancy. The results obtained from the above
experiment suggest that the conceptus evokes the immune responses locally as well
as systemically and that immune responses against the conceptus involve changes in
both the B cell and T cell systems, and especially the the T-cell system in early pregnancy
and the B-cell system in late pregnancy may be operating.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 32 No.4 (1991)
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