Immunohistochemical Study of the Distribution of Corticotropin Releasing Factor and Serotonin in the Cerebellar Nuclei of Monkeys

Abstract

Immunohistochemical methods, employing a specific antiserum against human corticotropin releasing factor (CRF) and serotonin, were applied to determine the distribution of corticotropin releasing factor-immunoreactivity (CRF-IR) and serotonin- IR in the deep cerebellar nuclei of the Squirrel monkey. CRF-IR labeled fibers were demonstrated in three all deep cerebellar nuclei, including the dentate, interposed and fastigial nucleus. All these fibers were varicose and greater density was observed in the dentate and the interposed nuclei. The serotonin-IR fibers were observed in all deep cerebellar nuclei. But the distribution of these fibers was denser than that of CRF-IR fibers. Labeled fibers were varicose, and there was no evidence of greater density within deep cerebellar nuclei.