Some VNTR Loci Typing from Single Hairs
두모 DNA에서 VNTR 유전자형 검색

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Lee, Jung Bin
Issue Date
Seoul National University College of Medicine
Seoul J Med 1991;32(4):209-215
PCRVNTRHairD1S80D17S30Apolipoprotein B gene
Hairs were taken from 11 families (57 individuals) and peripheral blood
was also drawn from 5 out of the 11 families (28 individuals). DNAs isolated from
the hairs and peripheral bloods were amplified by polymerase chain reaction (peR)
with primers of the D1580 locus, D17530 locus, and the 3' hypervariable region of
the apolipoprotein B gene. The fragment length polymorphism of amplified products
(AMP-FLP) was analyzed by acrylamide gel electrophoresis followed by ethidium bromide
staining. The recovery of DNA from a single hair was variable from person to
person and even in an individual, 174 nq/hair on average, which was sufficient for
analysis of the 3 genetic loci. The AMP-FLP of the hairs was identical to that of the
peripheral blood in all 3 genetic loci examined. The inheritance pattern of the 3 genetic
loci was analyzed in amplified products of hair DNA, demonstrating exclusively Mendelian
inheritance in all 11 families. Using hair DNAs of the 11 families, 15 alleles were
detected in the D1580 locus, 11 alleles in the D17530 locus, and 9 alleles in the 3'
hypervariable region of apolipoprotein B gene. Thus, analyses of genetic loci could
be performed using hair DNA instead of peripheral blood DNA, when an appropriate
amplification method is available and blood sampling is for some reason difficult.
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Medicine (의학과)The Seoul Journal of MedicineThe Seoul Journal of Medicine Vol. 32 No.4 (1991)
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