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Properties of novel thermostable glucoamylase from the hyperthermophilic archaea, Sulfolobus solfataricus, in relation to glucose production

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Authors
김미선
Advisor
박관화
Issue Date
2004
Publisher
서울대학교 대학원
Keywords
내열성 글루코아밀레이즈Thermostable glucoamylase설폴로버스 솔파타리쿠스Sulfolobus solfataricus포도당 생산Glucose production
Description
Thesis (master`s)--서울대학교 대학원 :농생명공학부,2004.
Abstract
The properties of a putative glucoamylase from a hyperthermophilic archaea, Sulfolobus
solfataricus (SSG), were examined in relation to the process of glucose syrup. The recombinant
glucoamylase was extremely thermostable showing its optimal temperature at 90°C. In addition,
the enzyme was most active in the pH range of 5.5 ? 6.0. In particular, substrate preference
toward maltotriose distinguished this enzyme from other fungal glucoamylase. The isomaltose
formation by reverse reaction was significantly reduced when compared to that of industrial
fungal glucoamylase (AMG) and the glucose yield was about 96%. To reduce the reverse reaction of
the enzyme, serine at the position 600 was replaced with alanine by site-directed mutagenesis.
S600A reduced the isomaltose formation by reverse reaction compared with the wild type
enzyme.Then, the glucoamylase from Sulfolobus solfataricus and its mutant enzyme S600A acting at
pH 6.0 and high temperature provides the excellent properties for improved industrial starch
process by eliminating the adjusting step of both pH and temperature.
Language
English
URI
http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000055146

http://hdl.handle.net/10371/67588
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College of Agriculture and Life Sciences (농업생명과학대학)Dept. of Food and Animal Biotechnology (식품·동물생명공학부)Theses (Master's Degree_식품·동물생명공학부)
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