Proteomic analysis of physiological properties of Candida magnoliae wild and its mutant strains

Abstract

Erythritol is a non-cariogenic, low-caloric (0.3 kcal/g) sweetener and safe for diabetics. The four-carbon sugar alcohol has about 70-80% the sweetness of sucrose and is found in fruits, mushrooms and some fermented foods. The objective of this research includes identification and analysis of the expression patterns of proteins between wild and its mutant strains of Candida magnoliae which was able to produce erythritol with high yield. In an attempt to identify up-regulated or down-regulated proteins twodimensional gel electrophoresis was performed for quantitative and qualitative comparison of total intracellular proteins. Most of the proteins were distributed in the range of pI 4-7 and MW 29-97 kD.