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Mechanism of humoral and cellular immune modulation provided by porcine sertoli cells

Cited 6 time in Web of Science Cited 7 time in Scopus
Authors
Lee, Hak-Mo; Oh, Byoung Chol; Lim, Dong-Pyo; Lee, Dong-Sup; Lim, Hong-Gook; Park, Chun Soo; Lee, Jeong Ryul
Issue Date
2008-06-28
Publisher
Korean Academy of Medical Sciences
Citation
J Korean Med Sci. 2008; 23(3): 514-20
Keywords
AnimalsAntibodies, Heterophile/immunologyAntibody Formation/*immunologyAntigens, CD40/immunologyAorta/cytologyCell Line, TransformedCell Survival/immunologyComplement Membrane Attack Complex/immunologyComplement System Proteins/immunologyDendritic Cells/cytology/immunologyEndothelial Cells/cytology/immunologyEpitopes/immunologyHumansImmune Tolerance/*immunologyImmunity, Cellular/*immunologyMaleMiceMice, Inbred C57BLSertoli Cells/cytology/*immunologySwine*Tissue EngineeringTransplantation, Heterologous
Abstract
The understanding of main mechanisms that determine the ability of immune privilege related to Sertoli cells (SCs) will provide clues for promoting a local tolerogenic environment. In this study, we evaluated the property of humoral and cellular immune response modulation provided by porcine SCs. Porcine SCs were resistant to human antibody and complement-mediated formation of the membrane attack complex (38.41+/-2.77% vs. 55.02+/-5.44%, p=0.027) and cell lysis (42.95+/-1.75% vs. 87.99 +/-2.25%, p<0.001) compared to immortalized aortic endothelial cells, suggesting that porcine SCs are able to escape cellular lysis associated with complement activation by producing one or more immunoprotective factors that may be capable of inhibiting membrane attack complex formation. On the other hand, porcine SCs and their culture supernatant suppressed the up-regulation of CD40 expression (p<0.05) on DCs in the presence of LPS stimulation. These novel findings, as we know, suggest that immune modulatory effects of porcine SCs in the presence of other antigen can be obtained from the first step of antigen presentation. These might open optimistic perspectives for the use of porcine SCs in tolerance induction eliminating the need for chronic immunosuppressive drugs.
ISSN
1011-8934 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18583891

http://hdl.handle.net/10371/67666
DOI
https://doi.org/10.3346/jkms.2008.23.3.514
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College of Medicine/School of Medicine (의과대학/대학원)Anatomy (해부학전공)Journal Papers (저널논문_해부학전공)
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