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Direct application of AvaII PCR restriction fragment length polymorphism analysis (AvaII PRA) targeting 644 bp heat shock protein 65 (hsp65) gene to sputum samples

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Authors
Mun, Ho-Suk; Kim, Hyun-Ju; Oh, Eun-Ju; Kim, Hong; Park, Young-Gil; Bai, Gil-Han; Do, Junghwan; Cha, Chang-Yong; Kook, Yoon-Hoh; Kim, Bum-Joon
Issue Date
2007-01-24
Publisher
Wiley-Blackwell
Citation
Microbiol Immunol. 51(1):105-110
Keywords
Bacterial Proteins/*geneticsBacteriological TechniquesChaperonins/*genetics*DNA FingerprintingDNA, Bacterial/*genetics/metabolismDeoxyribonucleases, Type II Site-Specific/metabolismHumansMycobacterium/genetics/*isolation & purificationPolymerase Chain Reaction*Polymorphism, Restriction Fragment LengthSensitivity and SpecificitySputum/*microbiology
Abstract
To evaluate the usefulness of the AvaII PRA method targeting 644-bp hsp65 gene for the direct detection of pathogenic mycobacteria from clinical specimens, we applied this method to 40 sputum samples and compared the results to those obtained by IS 6110 PCR. Although this method showed a sensitivity slightly lower than IS 6110 PCR (97.5% vs. 100%), it detected infections of M. avium complex (MAC) in two patients, which was not possible by IS 6110 PCR. We conclude that AvaII PRA is a highly effective method for directly detecting pathogenic mycobacteria in primary clinical specimens.
ISSN
0385-5600 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17237605

http://hdl.handle.net/10371/67899
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College of Medicine/School of Medicine (의과대학/대학원)Microbiology (미생물학전공)Journal Papers (저널논문_미생물학전공)
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