Publications
Detailed Information
Direct application of AvaII PCR restriction fragment length polymorphism analysis (AvaII PRA) targeting 644 bp heat shock protein 65 (hsp65) gene to sputum samples
Cited 0 time in
Web of Science
Cited 0 time in Scopus
- Authors
- Issue Date
- 2007-01-24
- Publisher
- Wiley-Blackwell
- Citation
- Microbiol Immunol. 51(1):105-110
- Keywords
- Bacterial Proteins/*genetics ; Bacteriological Techniques ; Chaperonins/*genetics ; DNA, Bacterial/*genetics/metabolism ; Deoxyribonucleases, Type II Site-Specific/metabolism ; Humans ; Mycobacterium/genetics/*isolation & purification ; Polymerase Chain Reaction ; Sensitivity and Specificity ; Sputum/*microbiology ; DNA Fingerprinting ; Polymorphism, Restriction Fragment Length
- Abstract
- To evaluate the usefulness of the AvaII PRA method targeting 644-bp hsp65 gene for the direct detection of pathogenic mycobacteria from clinical specimens, we applied this method to 40 sputum samples and compared the results to those obtained by IS 6110 PCR. Although this method showed a sensitivity slightly lower than IS 6110 PCR (97.5% vs. 100%), it detected infections of M. avium complex (MAC) in two patients, which was not possible by IS 6110 PCR. We conclude that AvaII PRA is a highly effective method for directly detecting pathogenic mycobacteria in primary clinical specimens.
- ISSN
- 0385-5600 (Print)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17237605
https://hdl.handle.net/10371/67899
- Files in This Item:
- There are no files associated with this item.
- Appears in Collections:
Item View & Download Count
Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.