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Effects of thiazide on the expression of TRPV5, calbindin-D28K, and sodium transporters in hypercalciuric rats

Cited 14 time in Web of Science Cited 16 time in Scopus
Authors
Jang, Hye Ryoun; Kim, Sejoong; Heo, Nam Ju; Lee, Jeong Hwan; Kim, Hyo Sang; Nielsen, Soren; Jeon, Un Sil; Oh, Yun Kyu; Na, Ki Young; Joo, Kwon Wook; Han, Jin Suk
Issue Date
2009-02-12
Publisher
Korean Academy of Medical Science
Citation
J Korean Med Sci. 2009;24 Suppl:S161-S169
Keywords
AnimalsBiological TransportCalcium/urineCalcium Channels/chemistryCalcium-Binding Protein, Vitamin D-Dependent/*biosynthesisHydrochlorothiazide/pharmacologyHypercalciuria/*therapyMaleModels, BiologicalRatsRats, Sprague-DawleySodium/*metabolismSodium-Hydrogen Antiporter/chemistrySodium-Potassium-Chloride Symporters/metabolismTRPV Cation Channels/*biosynthesis/chemistryThiazides/*pharmacology
Abstract
TRPV5 is believed to play an important role in the regulation of urinary calcium excretion. We assessed the effects of hydrochlorothiazide (HCTZ) on the expression of TRPV5, calbindin-D(28K), and several sodium transporters in hypercalciuric rats. Sprague-Dawley rats were divided into 4 groups; control, HCTZ, high salt, and high salt with HCTZ group in experiment 1; control, HCTZ, high calcium (Ca), and high Ca with HCTZ group in experiment 2. To quantitate the expression of TRPV5, calbindin-D(28K), and sodium transporters, western blotting was performed. In both experiments, HCTZ significantly decreased urinary calcium excretion. TRPV5 protein abundance decreased in all hypercalciuric rats, and restored by HCTZ in both high salt with HCTZ and high Ca with HCTZ group. Calbindin-D(28K) protein abundance increased in the high salt and high salt with HCTZ groups, but did not differ among groups in experiment 2. Protein abundance of NHE3 and NKCC2 decreased in all hypercalciuric rats, and were restored by HCTZ in only high Ca-induced hypercalciuric rats. In summary, protein abundance of TRPV5, NHE3, and NKCC2 decreased in all hypercalciuric rats. The hypocalciuric effect of HCTZ is associated with increased protein abundance of TRPV5 in high salt or calcium diet-induced hypercalciuric rats.
ISSN
1011-8934 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19194547

http://synapse.koreamed.org/Synapse/Data/PDFData/0063JKMS/jkms-24-S161.pdf

http://hdl.handle.net/10371/67935
DOI
https://doi.org/10.3346/jkms.2009.24.S1.S161
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College of Medicine/School of Medicine (의과대학/대학원)Internal Medicine (내과학전공)Journal Papers (저널논문_내과학전공)
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