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Identification of proteomic biomarkers of preeclampsia in amniotic fluid using SELDI-TOF mass spectrometry
Cited 43 time in
Web of Science
Cited 48 time in Scopus
- Authors
- Issue Date
- 2008-06-27
- Publisher
- SAGE Publications
- Citation
- Reprod Sci. 2008;15(5):457-468
- Keywords
- Adult ; Amniotic Fluid/*chemistry/metabolism ; Apolipoprotein A-I/*analysis ; Biological Markers/analysis ; Diagnosis, Differential ; Female ; Humans ; Hypertension/diagnosis/metabolism ; Infant, Newborn ; Pilot Projects ; Pre-Eclampsia/diagnosis/*metabolism ; Pregnancy ; Proteomics/*methods ; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods
- Abstract
- OBJECTIVE: To identify proteomic biomarkers in amniotic fluid (AF) that can distinguish preeclampsia (PE) from chronic hypertension (CHTN) and normotensive controls (CTR). METHODS: AF from women with PE, CHTN, and CTR were subjected to proteomic analysis by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Proteomic profiling of AF identified 2 biomarkers: peak X (17399.11 Da), which distinguished PE from CTR, and peak Y (28023.34 Da), which distinguished PE and CHTN from CTR. High performance liquid chromatography fractions containing the biomarkers were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in-gel tryptic digestion. The biomarkers were matched to proapolipoprotein A-I (peak Y) and a functionally obscure peptide, SBBI42 (peak X). Western blot analysis confirmed that AF from PE and CHTN had higher proapolipoprotein A-I levels than CTR. CONCLUSION: Proteomic analysis of AF can distinguish PE from CHTN and CTR. The discriminatory proteins were identified as proapolipoprotein A-I and SBBI42.
- ISSN
- 1933-7205 (Electronic)
- Language
- English
- URI
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18579854
http://rsx.sagepub.com/cgi/reprint/15/5/457.pdf
https://hdl.handle.net/10371/68095
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