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Transient receptor potential vanilloid type 1 receptor regulates glutamatergic synaptic inputs to the spinothalamic tract neurons of the spinal cord deep dorsal horn

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dc.contributor.authorKim, H-
dc.contributor.authorCui, L-
dc.contributor.authorKim, J-
dc.contributor.authorKim, S J-
dc.date.accessioned2010-07-01T05:37:39Z-
dc.date.available2010-07-01T05:37:39Z-
dc.date.issued2009-02-25-
dc.identifier.citationNeuroscience. 160(2):508-516en
dc.identifier.issn1873-7544 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19236908-
dc.identifier.urihttp://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T0F-4VNK6C2-1-N&_cdi=4861&_user=168665&_orig=search&_coverDate=05%2F05%2F2009&_sk=998399997&view=c&wchp=dGLzVlz-zSkWz&md5=a7907d5c04c5913dc476e9430febd705&ie=/sdarticle.pdf-
dc.identifier.urihttp://hdl.handle.net/10371/68131-
dc.description.abstractThe spinothalamic tract (STT) neurons in the spinal dorsal horn play an important role in transmission and processing of nociceptive sensory information. Although transient receptor potential vanilloid type 1 (TRPV1) receptors are present in the spinal cord dorsal horn, their physiological function is not fully elucidated. In this study, we examined the role of TRPV1 in modulating neuronal activity of the STT neurons through excitatory and inhibitory synaptic inputs. Whole-cell patch-clamp recordings were performed on STT neurons labeled by a retrograde fluorescent tracer injected into the ventral posterior lateral (VPL) nucleus of the thalamus. Capsaicin (1 microM) increased the frequency of miniature excitatory postsynaptic currents (mEPSC) without changing the amplitude or decay time constant of mEPSC. In contrast, capsaicin had no distinct effect on GABAergic miniature inhibitory postsynaptic currents (mIPSC). Capsazepine (10 microM), a TRPV1 receptor antagonist, abolished the effect of capsaicin on mEPSCs. Capsazepine itself did not affect the baseline amplitude and frequency of mEPSC. The effect of capsaicin on mEPSC was also abolished by removal of external Ca(2+), but not by treatment with Cd(2+). Furthermore, capsaicin increased the firing activity of the STT neurons and this increase in neuronal activity by capsaicin was abolished in the presence of non-N-methyl-d-aspartic acid (NMDA) and NMDA receptor antagonists, 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo[f]quinoxaline-2,3-dione (NBQX) and (R)-amino-5-phosphonovaleric acid (APV). These data suggest that activation of TRPV1 potentiates the glutamate release from excitatory terminals of primary afferent fibers and subsequently increases the neural activity of STT neurons of the rat spinal cord deep dorsal horn.en
dc.description.sponsorshipThis work was supported by a Korea Research
Foundation Grant funded by the Korea Government
(MOEHRD, Basic Research Promotion Fund, KRF-2005-015-
E00034) and a grant of the Korea Healthcare Technology R&D
Project, Ministry of Health, Welfare and Family Affairs (A080588).
en
dc.language.isoenen
dc.publisherElsevieren
dc.subjectAnimalsen
dc.subjectCalcium Channels/metabolismen
dc.subjectCapsaicin/pharmacologyen
dc.subjectExcitatory Postsynaptic Potentials/*physiologyen
dc.subjectFemaleen
dc.subjectGlutamic Acid/metabolismen
dc.subjectMaleen
dc.subjectMiniature Postsynaptic Potentials/physiologyen
dc.subjectPain/*metabolismen
dc.subjectPosterior Horn Cells/*metabolismen
dc.subjectRatsen
dc.subjectRats, Sprague-Dawleyen
dc.subjectSensory System Agents/pharmacologyen
dc.subjectSpinothalamic Tracts/cytology/*metabolismen
dc.subjectStatistics, Nonparametricen
dc.subjectSynaptic Transmission/*physiologyen
dc.subjectTRPV Cation Channels/*metabolismen
dc.subjectgamma-Aminobutyric Acid/metabolismen
dc.titleTransient receptor potential vanilloid type 1 receptor regulates glutamatergic synaptic inputs to the spinothalamic tract neurons of the spinal cord deep dorsal hornen
dc.typeArticleen
dc.identifier.doi10.1016/j.neuroscience.2009.02.019-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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