Publications

Detailed Information

Involvement of phosphatidylinositol 4,5-bisphosphate in the desensitization of canonical transient receptor potential 5

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors

Kim, Byung Joo; Kim, Min Tae; Jeon, Ju-Hong; Kim, Seon Jeong; So, Insuk

Issue Date
2008-09-02
Publisher
Pharmaceutical Society of Japan
Citation
Biol Pharm Bull. 31(9), 1733-1738
Keywords
Actins/metabolismAnimalsCarbachol/pharmacologyCell Line, TumorCell SeparationCytoskeleton/drug effects/metabolismFemaleHumansIleum/cytology/drug effectsMaleMiceMice, Inbred ICRMuscarinic Agonists/pharmacologyMyocytes, Smooth Muscle/drug effects/metabolismPatch-Clamp TechniquesPhosphatidylinositol 4,5-Diphosphate/metabolism/*physiologyTRPC Cation Channels/*drug effects/metabolismTransfection
Abstract
The classic transient receptor potential channel (TRPC) is a candidate for Ca(2+)-permeable cation channel in mammalian cells. TRPC5 is desensitized rapidly after activation by G protein-coupled receptor. Here we investigate the mechanisms of desensitization of TRPC5 using patch-clamp recording. TRPC5 was initially activated by muscarinic stimulation using 50 microM carbachol (CCh) and decayed rapidly in the presence of CCh (desensitization). Intracellularly-applied phosphatidylinositol 4,5-bisphosphate (PIP(2)) slowed the rate of desensitization. In contrast, several other phosphoinositides, including PI(3,4)P(2), PI(3,5)P(2), PI(3,4,5)P(3) and PI(4)P, had no effect on the desensitization of the TRPC5 current. This indicates that PIP(2) attenuates the desensitization of the TRPC5 current in a highly selective manner. Neither wortmannin, an inhibitor of phosphatidylinositol 4-kinase, or poly-L-lysine (PLL), a scavenger of PIP(2), had any effect on desensitization of the TRPC5 current. PIP(2) breakdown appears to be a required step in the desensitization of TRPC5 current, but PIP(2) depletion alone was insufficient for channel desensitization. TRPC5 was inhibited by cytochalasin D treatment. In mouse ileal myocytes, the desensitization of CCh-activated inward current (I(CCh)) also slowed in the presence of PIP(2) in recording pipettes. These results indicate that PIP(2) is involved in the desensitization of TRPC5 currents.
ISSN
0918-6158 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18758068

http://www.jstage.jst.go.jp/article/bpb/31/9/1733/_pdf

https://hdl.handle.net/10371/68141
Files in This Item:
There are no files associated with this item.
Appears in Collections:

Altmetrics

Item View & Download Count

  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Share