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Ghrelin enhances the proliferating effect of thyroid stimulating hormone in FRTL-5 thyroid cells

Cited 21 time in Web of Science Cited 20 time in Scopus
Authors
Park, Young Joo; Lee, You Jin; Kim, Soon Hee; Joung, Duck Soon; Kim, Byung Joo; So, Insuk; Park, Do Joon; Cho, Bo Youn
Issue Date
2008-03-04
Publisher
Elsevier
Citation
Mol Cell Endocrinol. 285(1-2), 19-25
Keywords
Adaptor Proteins, Signal Transducing/genetics/metabolismAnimalsCalcium/metabolismCell Line*Cell ProliferationCyclic AMP/metabolismEnzyme ActivationExtracellular Signal-Regulated MAP Kinases/metabolismGhrelin/genetics/*metabolismHumansProto-Oncogene Proteins c-akt/metabolismRatsReceptors, Ghrelin/genetics/*metabolismSTAT3 Transcription Factor/metabolismShc Signaling Adaptor ProteinsSignal Transduction/physiologyThyroid Gland/*cytology/metabolismThyrotropin/*metabolism
Abstract
Ghrelin regulates cell proliferation through the growth hormone secretagogue receptor (GHS-R). We confirmed the expression of GHS-R in FRTL-5 thyroid cells and investigated the effects of ghrelin in thyrocytes using FRTL-5 cells. Ghrelin increased intracellular calcium levels but not intracellular cyclic AMP levels. Ghrelin activated Erk within 2min, then activated Akt and STAT3. Erk phosphorylation was inhibited by the calcium inhibitor cyclopiazonic acid (CPA). Ghrelin alone did not stimulate FRTL-5 cell proliferation but enhanced the effects of thyroid stimulating hormone (TSH). Pretreatment with TSH potentiates the growth effects of ghrelin in thyroid cells, and p66Shc, a growth factor receptor adaptor protein, might mediate these synergistic effects. Ghrelin phosphorylated TSH-induced p66Shc, which was inhibited by CPA. Ghrelin did not affect the proliferation of ARO cells, which showed no increased expression of p66Shc after TSH treatment. Thus, ghrelin-induced intracellular calcium signaling enhanced the TSH-induced proliferation of thyrocytes, possibly mediated by the p66Shc pathway.
ISSN
0303-7207 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18313206

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T3G-4RM7N3C-2-3&_cdi=4946&_user=168665&_orig=search&_coverDate=03%2F26%2F2008&_sk=997149998&view=c&wchp=dGLzVtz-zSkWA&md5=7d4911efb4c606529ef9309221317897&ie=/sdarticle.pdf

http://hdl.handle.net/10371/68175
DOI
https://doi.org/10.1016/j.mce.2008.01.003
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Physiology (생리학교실)Journal Papers (저널논문_생리학교실)
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