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Susceptibility of porcine keratocytes to immune-mediated damage in xeno-related rejection

Cited 7 time in Web of Science Cited 7 time in Scopus
Authors
Kim, M K; Oh, J Y; Lee, H I; Ko, J H; Lee, H J; Lee, J H; Wee, W R
Issue Date
2008-04-01
Publisher
Elsevier
Citation
Transplant Proc. 40(2):564-569
Keywords
AnimalsAntibody-Dependent Cell CytotoxicityCells, CulturedCornea/*cytology/*immunologyCorneal Transplantation/*immunologyDNA PrimersEndothelial Cells/*cytology/*immunologyEndothelins/geneticsGraft Rejection/*immunology/pathologyHumansReverse Transcriptase Polymerase Chain ReactionSwineTransplantation, Heterologous/*immunology/pathologyVimentin/genetics
Abstract
We admixed cultured porcine keratocytes or corneal endothelial cells in the presence of human sera or peripheral blood mononuclear cells (PBMCs) for 4 to 72 hours to investigate their immune-related susceptibilities to xeno-related rejection. We evaluated complement deposition at 48 hours by flow cytometry after staining with the C3 anti-goat cy3 antibody. The inhibition of proliferation of porcine corneal cells by human sera was examined using the 3-[4,5-dimethy/thiazol-2,5-dephenyl tetrazolium bromide (MTT) assay over 24 to 72 hours. The amount of 51chromium (Cr)-release was estimated after a reaction between the porcine cells and human PBMCs for 4 hours. There was greater C3 deposition in keratocytes (60.2%) than in endothelial cells (26.9%; P = .05, Mann-Whitney U test). Both keratocytes and endothelial cells showed significant levels of proliferative inhibition over a period of 72 hours. The number of 51Cr-release cells on interleukin-2 addition was significantly higher among keratocytes (88.0%) than endothelial cells (51.4%) at a 1:100 target:effector ratio (P = .04, Mann-Whitney U test). Our present data suggested that porcine keratocytes might be key target cells in xeno-related rejections when the porcine cornea is transplanted to primates.
ISSN
0041-1345 (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18374130

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6VJ0-4S4SK83-2J-9&_cdi=6080&_user=168665&_orig=search&_coverDate=03%2F31%2F2008&_sk=999599997&view=c&wchp=dGLbVzb-zSkWb&md5=30591250bbf2f3ac76f6c25a0f421d2a&ie=/sdarticle.pdf

http://hdl.handle.net/10371/68228
DOI
https://doi.org/10.1016/j.transproceed.2007.12.024
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College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
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