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The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Oh, Joo Youn | - |
dc.contributor.author | Kim, Mee Kum | - |
dc.contributor.author | Shin, Mi Sun | - |
dc.contributor.author | Lee, Hyun Ju | - |
dc.contributor.author | Ko, Jung Hwa | - |
dc.contributor.author | Wee, Won Ryang | - |
dc.contributor.author | Lee, Jin Hak | - |
dc.date.accessioned | 2010-07-05T03:48:57Z | - |
dc.date.available | 2010-07-05T03:48:57Z | - |
dc.date.issued | 2008-01-15 | - |
dc.identifier.citation | Stem Cells. 2008;26(4):1047-1055 | en |
dc.identifier.issn | 1549-4918 (Electronic) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18192235 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68256 | - |
dc.description.abstract | To investigate the anti-inflammatory and anti-angiogenic effects of mesenchymal stem cells (MSC) in the chemically burned corneas, we mechanically removed the corneal epithelium of rats after 100% alcohol instillation. The rats were then randomized into four groups: fresh media, conditioned media derived from the MSC culture (MSC-CM), MSC applied topically to the damaged corneas for 2 hours immediately after the injury or MSC-CM applied either once or 3 times per day for 3 consecutive days. Corneal surface was evaluated every week. After 3 weeks, the corneas were stained with the hematoxylin-eosin, and the expression of interleukin (IL)-2, interferon (IFN)-gamma, IL-6, IL-10, transforming growth factor (TGF)-beta1, thrombospondin-1 (TSP-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor (VEGF) were analyzed. CD4+ cells were assessed in the corneas. We found that both MSC and three-time applied MSC-CM (1) reduced corneal inflammation and neovascularization, (2) decreased IL-2 and IFN-gamma, although increased IL-10 and TGF-beta1 as well as IL-6, (3) reduced the infiltration of CD4+ cells, and (4) upregulated the expression of TSP-1, although downregulated that of MMP-2. Interestingly, whereas three-time application of MSC-CM was partially effective, transplantation of MSC achieved a better outcome in suppressing corneal inflammation. The results of this study suggest that the anti-inflammatory and anti-angiogenic action of MSC in the chemically burned corneas might be mediated in part through paracrine pathways involving soluble factors such as IL-10, TGF-beta1, IL-6 and TSP-1. | en |
dc.language.iso | en | en |
dc.publisher | Wiley-Blackwell | en |
dc.subject | Animals | en |
dc.subject | Burns, Chemical/pathology/*surgery | en |
dc.subject | Cell Line | en |
dc.subject | Cells, Cultured | en |
dc.subject | Cornea/*injuries/pathology/surgery | en |
dc.subject | Inflammation/pathology/prevention & control/surgery | en |
dc.subject | Male | en |
dc.subject | Mesenchymal Stem Cell Transplantation/*methods | en |
dc.subject | Neovascularization, Pathologic/chemically induced/pathology/*surgery | en |
dc.subject | Rats | en |
dc.subject | Rats, Sprague-Dawley | en |
dc.subject | Wound Healing/*physiology | en |
dc.title | The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 오주연 | - |
dc.contributor.AlternativeAuthor | 김미금 | - |
dc.contributor.AlternativeAuthor | 신미선 | - |
dc.contributor.AlternativeAuthor | 이현주 | - |
dc.contributor.AlternativeAuthor | 고정화 | - |
dc.contributor.AlternativeAuthor | 위원량 | - |
dc.contributor.AlternativeAuthor | 이진학 | - |
dc.identifier.doi | 10.1634/stemcells.2007-0737 | - |
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