Browse

The anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injury

DC Field Value Language
dc.contributor.authorOh, Joo Youn-
dc.contributor.authorKim, Mee Kum-
dc.contributor.authorShin, Mi Sun-
dc.contributor.authorLee, Hyun Ju-
dc.contributor.authorKo, Jung Hwa-
dc.contributor.authorWee, Won Ryang-
dc.contributor.authorLee, Jin Hak-
dc.date.accessioned2010-07-05T03:48:57Z-
dc.date.available2010-07-05T03:48:57Z-
dc.date.issued2008-01-15-
dc.identifier.citationStem Cells. 2008;26(4):1047-1055en
dc.identifier.issn1549-4918 (Electronic)-
dc.identifier.urihttp://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18192235-
dc.identifier.urihttp://hdl.handle.net/10371/68256-
dc.description.abstractTo investigate the anti-inflammatory and anti-angiogenic effects of mesenchymal stem cells (MSC) in the chemically burned corneas, we mechanically removed the corneal epithelium of rats after 100% alcohol instillation. The rats were then randomized into four groups: fresh media, conditioned media derived from the MSC culture (MSC-CM), MSC applied topically to the damaged corneas for 2 hours immediately after the injury or MSC-CM applied either once or 3 times per day for 3 consecutive days. Corneal surface was evaluated every week. After 3 weeks, the corneas were stained with the hematoxylin-eosin, and the expression of interleukin (IL)-2, interferon (IFN)-gamma, IL-6, IL-10, transforming growth factor (TGF)-beta1, thrombospondin-1 (TSP-1), matrix metalloproteinase-2 (MMP-2), and vascular endothelial growth factor (VEGF) were analyzed. CD4+ cells were assessed in the corneas. We found that both MSC and three-time applied MSC-CM (1) reduced corneal inflammation and neovascularization, (2) decreased IL-2 and IFN-gamma, although increased IL-10 and TGF-beta1 as well as IL-6, (3) reduced the infiltration of CD4+ cells, and (4) upregulated the expression of TSP-1, although downregulated that of MMP-2. Interestingly, whereas three-time application of MSC-CM was partially effective, transplantation of MSC achieved a better outcome in suppressing corneal inflammation. The results of this study suggest that the anti-inflammatory and anti-angiogenic action of MSC in the chemically burned corneas might be mediated in part through paracrine pathways involving soluble factors such as IL-10, TGF-beta1, IL-6 and TSP-1.en
dc.language.isoenen
dc.publisherWiley-Blackwellen
dc.subjectAnimalsen
dc.subjectBurns, Chemical/pathology/*surgeryen
dc.subjectCell Lineen
dc.subjectCells, Cultureden
dc.subjectCornea/*injuries/pathology/surgeryen
dc.subjectInflammation/pathology/prevention & control/surgeryen
dc.subjectMaleen
dc.subjectMesenchymal Stem Cell Transplantation/*methodsen
dc.subjectNeovascularization, Pathologic/chemically induced/pathology/*surgeryen
dc.subjectRatsen
dc.subjectRats, Sprague-Dawleyen
dc.subjectWound Healing/*physiologyen
dc.titleThe anti-inflammatory and anti-angiogenic role of mesenchymal stem cells in corneal wound healing following chemical injuryen
dc.typeArticleen
dc.contributor.AlternativeAuthor오주연-
dc.contributor.AlternativeAuthor김미금-
dc.contributor.AlternativeAuthor신미선-
dc.contributor.AlternativeAuthor이현주-
dc.contributor.AlternativeAuthor고정화-
dc.contributor.AlternativeAuthor위원량-
dc.contributor.AlternativeAuthor이진학-
dc.identifier.doi10.1634/stemcells.2007-0737-
Appears in Collections:
College of Medicine/School of Medicine (의과대학/대학원)Ophthalmology (안과학전공)Journal Papers (저널논문_안과학전공)
Files in This Item:
There are no files associated with this item.
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse