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MCP-1/CCR2 system is involved in high glucose-induced fibronectin and type IV collagen expression in cultured mesangial cells
DC Field | Value | Language |
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dc.contributor.author | Park, Jehyun | - |
dc.contributor.author | Ryu, Dong-Ryeol | - |
dc.contributor.author | Li, Jin Ji | - |
dc.contributor.author | Jung, Dong-Sub | - |
dc.contributor.author | Kwak, Seung-Jae | - |
dc.contributor.author | Lee, Sun Ha | - |
dc.contributor.author | Yoo, Tae-Hyun | - |
dc.contributor.author | Han, Seung Hyeok | - |
dc.contributor.author | Lee, Jung Eun | - |
dc.contributor.author | Kim, Dong Ki | - |
dc.contributor.author | Moon, Sung Jin | - |
dc.contributor.author | Kim, Kunhong | - |
dc.contributor.author | Han, Dae Suk | - |
dc.contributor.author | Kang, Shin-Wook | - |
dc.date.accessioned | 2010-07-05T03:55:15Z | - |
dc.date.available | 2010-07-05T03:55:15Z | - |
dc.date.issued | 2008-06-27 | - |
dc.identifier.citation | Am J Physiol Renal Physiol. 295(3): F749-F757 | en |
dc.identifier.issn | 0363-6127 (Print) | - |
dc.identifier.uri | http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18579703 | - |
dc.identifier.uri | http://ajprenal.physiology.org/cgi/reprint/295/3/F749.pdf | - |
dc.identifier.uri | https://hdl.handle.net/10371/68261 | - |
dc.description.abstract | Monocyte chemoattractant protein-1 (MCP-1) is a potent chemokine that plays an important role in the recruitment of macrophages. Although previous studies have demonstrated the importance of MCP-1 in the pathogenesis of diabetic nephropathy (DN) in terms of inflammation, the role of MCP-1 and its receptor (C-C chemokine receptor 2; CCR2) in extracellular matrix (ECM) accumulation under diabetic conditions has been largely unexplored. This study was undertaken to investigate the functional role of the MCP-1/CCR2 system in high glucose-induced ECM (fibronectin and type IV collagen) protein expression in cultured mesangial cells (MCs). Mouse MCs were exposed to medium containing 5.6 mM glucose (NG), NG+24.4 mM mannitol (NG+M), or 30 mM glucose (HG) with or without mutant MCP-1 (mMCP-1), CCR2 small interfering (si)RNA, or CCR2 inhibitor (RS102895). To examine the relationship between MCP-1 and transforming growth factor (TGF)-beta1, MCs were also treated with TGF-beta1 (2 ng/ml) with or without mMCP-1 or CCR2 siRNA. Transient transfection was performed with Lipofectamine 2000 for 24 h. Cell viability was determined by an MTT assay, mouse and human MCP-1 and TGF-beta1 levels by ELISA, and CCR2 and ECM protein expression by Western blotting. Transfections of mMCP-1 and CCR2 siRNA increased human MCP-1 levels and inhibited CCR2 expression, respectively. HG-induced ECM protein expression and TGF-beta1 levels were significantly attenuated by mMCP-1, CCR2 siRNA, and RS102895 (P < 0.05). MCP-1 directly increased ECM protein expression, and this increase was inhibited by an anti-TGF-beta1 antibody. In addition, TGF-beta1-induced ECM protein expression was significantly abrogated by the inhibition of the MCP-1/CCR2 system (P < 0.05). These results suggest that an interaction between the MCP-1/CCR2 system and TGF-beta1 may contribute to ECM accumulation in DN. | en |
dc.description.sponsorship | This work was supported by the BK21 (Brain Korea 21) Project for Medical
Sciences, Yonsei University, a faculty research grant from Yonsei University College of Medicine for 2007 (6-2007-0174), a Korea Research Foundation Grant funded by the Korean government (MOEHRD; KRF-2007-331- E00086), and Korea Science and Engineering Foundation (KOSEF) grants funded by the Korea government (MOST; R01-2007-000-20263-0 and R13- 2002-054-04001-0). | en |
dc.language.iso | en | en |
dc.publisher | American Physiological Society | en |
dc.subject | Animals | en |
dc.subject | Cells, Cultured | en |
dc.subject | Chemokine CCL2/genetics/*metabolism | en |
dc.subject | Collagen Type IV/*metabolism | en |
dc.subject | Diabetic Nephropathies/metabolism | en |
dc.subject | Fibronectins/*metabolism | en |
dc.subject | Glucose/metabolism | en |
dc.subject | Humans | en |
dc.subject | Mesangial Cells/*metabolism | en |
dc.subject | Mice | en |
dc.subject | Mice, Transgenic | en |
dc.subject | Mutation | en |
dc.subject | RNA, Small Interfering/genetics | en |
dc.subject | Receptors, CCR2/genetics/*metabolism | en |
dc.subject | Transfection | en |
dc.subject | Transforming Growth Factor beta1/metabolism | en |
dc.title | MCP-1/CCR2 system is involved in high glucose-induced fibronectin and type IV collagen expression in cultured mesangial cells | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 박제현 | - |
dc.contributor.AlternativeAuthor | 류동렬 | - |
dc.contributor.AlternativeAuthor | 이진지 | - |
dc.contributor.AlternativeAuthor | 정동섭 | - |
dc.contributor.AlternativeAuthor | 곽승재 | - |
dc.contributor.AlternativeAuthor | 이선하 | - |
dc.contributor.AlternativeAuthor | 유태현 | - |
dc.contributor.AlternativeAuthor | 한승혁 | - |
dc.contributor.AlternativeAuthor | 이정은 | - |
dc.contributor.AlternativeAuthor | 김동기 | - |
dc.contributor.AlternativeAuthor | 문성진 | - |
dc.contributor.AlternativeAuthor | 김근홍 | - |
dc.contributor.AlternativeAuthor | 한대석 | - |
dc.contributor.AlternativeAuthor | 강신욱 | - |
dc.identifier.doi | 10.1152/ajprenal.00547.2007 | - |
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