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Endogenous neurogenesis and neovascularization in the neocortex of the rat after focal cerebral ischemia

Cited 31 time in Web of Science Cited 37 time in Scopus
Authors
Shin, Hye Young; Kim, Jin Hyun; Phi, Ji Hoon; Park, Chul-Kee; Kim, Jung Eun; Kim, Jong-Hoon; Paek, Sun Ha; Wang, Kyu-Chang; Kim, Dong Gyu
Issue Date
2007-09-26
Publisher
Wiley-Blackwell
Citation
J Neurosci Res. 86(2):356-367
Keywords
AnimalsBlotting, WesternBrain Ischemia/*pathologyCell ProliferationGlial Fibrillary Acidic Protein/metabolismImmunohistochemistryIntermediate Filament Proteins/metabolismMaleMicroscopy, ConfocalNeocortex/blood supply/*physiologyNeovascularization, Physiologic/*physiologyNerve Regeneration/*physiologyNerve Tissue Proteins/metabolismRatsRats, Sprague-DawleyStem Cells/cytology/metabolism
Abstract
The present study was designed to examine whether endogenous neurogenesis and neovascularization occur in the neocortex of the ischemic rat brain after unilateral middle cerebral artery occlusion (MCAO). Sprague-Dawley rats were divided into six groups (n = 29): one control group (n = 4) and five groups composed of animals sacrificed at increasing times post-MCAO (2 days and 1, 2, 4, and 8 weeks; n = 5 per group). To determine the presence of neurogenesis and neovascularization in the ischemic brain, nestin, Tuj1, NeuN, GFAP, Tie2, RECA, and 5-bromo-2'-deoxyuridine (BrdU) were analyzed immunohistochemically. In addition, nestin, GFAP, and Tie2 expression was determined by Western blotting. Triple-labeling of nestin, BrdU, and laminin was performed to visualize the interaction between endogenous neurogenesis and neovascularization. The number of BrdU- and nestin-colabeled cells increased markedly in the neocortex and border zone of the ischemic area up to 1 week after MCAO and decreased thereafter. Western blot analysis revealed that the expression of nestin, Tie-2, and GFAP was amplified in the ipsilateral hemisphere 2 days after MCAO and peaked 1 week after MCAO, compared with that in the normal brain. After ischemic injury, nestin- and BrdU-colabeled cells were observed in the vicinity of the endothelial cells lining cerebral vessels in the ipsilateral neocortex of the ischemic brain. Endogenous neurogenesis and neovascularization were substantially activated and occurred in close proximity to one other in the ipsilateral neocortex of the ischemic rat brain.
ISSN
1097-4547 (Electronic)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17893912

http://hdl.handle.net/10371/68442
DOI
https://doi.org/10.1002/jnr.21494
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College of Medicine/School of Medicine (의과대학/대학원)Neurosurgery (신경외과학전공)Journal Papers (저널논문_신경외과학전공)
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