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Enforced physical training promotes neurogenesis in the subgranular zone after focal cerebral ischemia

Cited 16 time in Web of Science Cited 17 time in Scopus
Authors
Lee, Seung-Hoon; Kim, Yun-Hee; Kim, Young-Ju; Yoon, Byung-Woo
Issue Date
2008-02-12
Publisher
Elsevier
Citation
J Neurol Sci. 2008;269(1-2):54-61
Keywords
AnimalsBromodeoxyuridine/metabolismCell Count/methods*Cell ProliferationCerebral Ventricles/pathologyDentate Gyrus/*pathologyDisease Models, AnimalInfarction, Middle Cerebral Artery/*pathology/*rehabilitationMaleNerve Tissue Proteins/metabolismNeurologic ExaminationNeurons/*physiologyPhysical Conditioning, Animal/*methodsRatsRats, Sprague-DawleyTime Factors
Abstract
BACKGROUND: Cerebral ischemia increases neurogenesis in the subventricular zone (SVZ) and in the subgranular zone (SGZ) of the dentate gyrus, and this might be modulated by an enriched environment including voluntary physical activity. We examined whether enforced physical training (EPT) influences neurogenesis in the SVZ and SGZ after cerebral ischemia. METHODS: Adult male Sprague-Dawley rats were subjected to focal cerebral ischemia for 2 h, and divided into an EPT and a non-EPT group. All rats in the EPT group were trained using a rota-rod for 14 days. 5-bromo-2'-deoxyuridine (BrdU) was injected to determine levels of cell proliferation. Functional recovery was assessed using a set of behavioral test batteries. Extents of endogenous neurogenesis in the SVZ and SGZ were quantified by immunofluorescence staining. Although final infarction volumes were not significantly different in the groups, functional recovery was better in the EPT group at 10 and 17 days after ischemia. In the SVZ, BrdU labeling and double labeling of BrdU/Dcx and of BrdU/NeuN were not significantly different in the two groups. However, in the SGZ, EPT significantly increased the number of BrdU-positive cell numbers (EPT vs. non-EPT: 159.1+/-19.9 vs. 101.8+/-7.8, p=0.04), and the number of BrdU/Dcx double-labeled cells (130.6+/-16.9 vs. 73.6+/-7.2, p=0.01). CONCLUSIONS: The results obtained indicate that EPT promotes neurogenesis in the SGZ of the dentate gyrus after ischemia, but not in the SVZ. The biochemical mechanism that determines the differential effects of EPT remains to be clarified.
ISSN
0022-510X (Print)
Language
English
URI
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18262568

http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T06-4RTCPXY-1-F&_cdi=4854&_user=168665&_orig=search&_coverDate=06%2F15%2F2008&_sk=997309998&view=c&wchp=dGLzVlz-zSkzS&md5=6e424077596485fea1719e405d6dfed6&ie=/sdarticle.pdf

http://hdl.handle.net/10371/68445
DOI
https://doi.org/10.1016/j.jns.2007.12.028
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College of Medicine/School of Medicine (의과대학/대학원)Dept. of Neurology (신경과학교실)Journal Papers (저널논문_신경과학교실)
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