S-Space College of Agriculture and Life Sciences (농업생명과학대학) Dept. of Food and Animal Biotechnology (식품·동물생명공학부) Theses (Master's Degree_식품·동물생명공학부)
Genomic structure of amorpha-4, 11-diene synthase in artemisia annua L.
개똥쑥에서 amorpha-4, 11-diene synthase의 유전체 구조
- Issue Date
- 서울대학교 대학원
- 개똥쑥; Artemisia annua; 항말라리아성 물질; Antimalarial sesquiterpene; 프로모터의 유용인자; Artemisinin; 인트론; Amorpha-4; 11-diene synthase (ads); Promoter elements; Intron
- Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003.
- Artemisia annua, an indigenous plant to Korea, accumulates an antimalarial sesquiterpene,
artemisinin. Amorpha-4,11-diene synthase (ADS) is a sesquiterpene synthase in artemisinin
biosynthesis pathway. Open reading frame of ADS gene is described previously. To compose genomic
structure of ADS, 5''-upstream region and introns of ADS gene were found. Firstly, to search the
useful elements of ADS gene promoter, 5''-upstream region of 502 bp-long was isolated from the
translation start codon. Promoter elements of TATA box, H box, G box, BOX IV were found in 5''-
upstream region of ADS gene. Secondly, five pairs of primers were designed from the expected
regions of open reading frame of the synthase gene, and the original PCR amplicons of genomic
DNA were sequenced directly. Six introns, which were mostly same as those reported from Chinese
race were found. However, 1,729 bp-long second intron had an extra sequence of 34 bp compared to
the Chinese race. The discordance of the second intron of amorpha-4,11-diene synthase between
regional races of A. annua inhabiting China and Korea is discussed in the aspect of evolutionary
and functional implications.
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