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Physicochemical characteristics of maltosyl-trehalose, a transglycosylation product of trehalose by bacillus stearothermophilus maltogenic amylase : Trehalose의 당전이 산물인 maltoyl-trehalose의 이화학적 특성 구명
DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | 문태화 | - |
dc.contributor.author | 정일헌 | - |
dc.date.accessioned | 2010-07-08T23:16:48Z | - |
dc.date.available | 2010-07-08T23:16:48Z | - |
dc.date.copyright | 2003. | - |
dc.date.issued | 2003 | - |
dc.identifier.uri | http://dcollection.snu.ac.kr:80/jsp/common/DcLoOrgPer.jsp?sItemId=000000057809 | - |
dc.identifier.uri | https://hdl.handle.net/10371/68527 | - |
dc.description | Thesis (master`s)--서울대학교 대학원 :농생명공학부,2003. | en |
dc.description.abstract | physiological characteristics of the existing sugars. Maltogenic amylase from
Bacillus stearothermophilus (BSMA) catalyzes the transglycosylation reaction in the presence of suitable glycosyl acceptors. In this study, production of maltosyl-trehalose by transglycosylation reaction, structural determination of transferred product, and characterization of physicochemical properties were carried out. Maltosyl-trehalose was produced by maltogenic amylase from Bacillus stearothermphilus through the reaction with maltotriose as the maltosyl donor and trehalose as its acceptor. It was identified by thin layer chromatography and high performance ion chromatography. The transfer product was purified with Bio-Gel P-2 column chromatography and recycled prep high pressure liquid chromatography. The structure was identified by 13C-NMR analysis, matrixassisted laser desorption ionization with time-of-flight/mass spectrometry and liquid chromatography-mass spectrometry. Chemical shift of maltosyl-trehalose in 13C-NMR spectrum was compared with that of the trehalose. The chemical shift of one of two C-6 in trehalose changed from 61.39 to 44.48 and 66.78 for maltosyl-trehalose. The molecular mass was estimated to be 666 daltons from the MALDI-TOF analysis. These results support that the transglycosylated product was maltosyl-trehalose, in which maltose was attached by an ¥á-(1, 6) glycosidic linkage to trehalose. Optical rotation ([¥á]D) of maltosyl-trehalose was +155.7¢ª at 20¡É. Flow behavior of 10, 20, and 30% solution of maltosyl-trehalose showed typical Newtonian fluid characteristics. Sorption isotherm of maltosyl-trehalose displayed a typical type¥² isotherm curve. Glass-transition temperatures of maximally freezeconcentrated solutes (T''g), and T''m were observed at -49 and -42¡É, respectively. It also exhibited medium hygroscopicity and very low Maillard reactivity. | en |
dc.format.extent | viii, 43 leaves | en |
dc.language.iso | en | en |
dc.publisher | 서울대학교 대학원 | en |
dc.subject | Trehalose | en |
dc.subject | Trehalose | en |
dc.subject | 전이반응 | en |
dc.subject | Transglycosylation | en |
dc.subject | Maltosyl-trehalose | en |
dc.subject | Maltosyl-trehalose | en |
dc.subject | Bacillus stearothermophillus maltogenic amylase (BSMA) | en |
dc.subject | Bacillus stearothermophillus maltogenic amylase (bsma) | en |
dc.title | Physicochemical characteristics of maltosyl-trehalose, a transglycosylation product of trehalose by bacillus stearothermophilus maltogenic amylase | en |
dc.title.alternative | Trehalose의 당전이 산물인 maltoyl-trehalose의 이화학적 특성 구명 | en |
dc.type | Thesis | en |
dc.contributor.department | 농생명공학부 | - |
dc.description.degree | Master | en |
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