Browse

A role of sodium bicarbonate cotransporter(NBC) in HCO3- formation in human salivary gland acinar cells

Cited 0 time in Web of Science Cited 0 time in Scopus
Authors
Jin, Meehyun; Koo, Na-Youn; Jin, MeiHong; Hwang, Sung-Min; Park, Kyungpyo
Issue Date
2008
Publisher
The Korean Academy of Oral Biology
Citation
International Journal of Oral Biology, 2008;33(4):143-147
Keywords
sodium bicarbonate cotransporter (NBC)human parotid glandpHi recovery4'-diisothio cyanatostilbene-22'-disulphonic acid (DIDS)acetazolamide
Abstract
The sodium bicarbonate cotransporter (NBC) protein is functionally expressed in salivary glands. In this experiment, we examined the role of NBC in HCO3- formation in human parotid gland acinar cells. Intracellular pH (pHi) was measured in 2'-7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF) -loaded cells. Acetazolamide (0.1 mM) and 4,4'-diisothiocyanatostilbene-2,2'-disulphonic acid (DIDS, 0.5 mM) were used as specific inhibitors of carbonic anhydrase and NBC, respectively. The degree of inhibition was assessed by measuring the pHi recovery rate (△pHi/min) after cell acidification using an ammonium prepulse technique. In control experiments, △pHi/min was 1.40 ± 0.06. Treatment of cells with 0.5 mM DIDS or 0.1 mM acetazolamide significantly reduced △pH/min to 1.14 ± 0.14 and 0.74 ± 0.15, respectively. Simultaneous application of DIDS and acetazolamide further reduced △ pHi/min to 0.47 ± 0.10. Therefore, DIDS and acetazolamide reduced △pHi/min by 19 % and 47 %, respectively, while simultaneous application of both DIDS and acetazolamide caused a reduction in △pHi/min of 67 %. These results suggest that in addition to carbonic anhydrase, NBC also partially contributes to HCO3- formation in human parotid gland acinar cells.
ISSN
1226-7155
Language
Korean
URI
http://hdl.handle.net/10371/68588
Files in This Item:
Appears in Collections:
College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
  • mendeley

Items in S-Space are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse