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Effect of immobilized cell-binding peptides on chitosan membranes for osteoblastic differentiation of mesenchymal stem cells

Cited 18 time in Web of Science Cited 23 time in Scopus
Authors
Lee, Jue-Yeon; Choo, Jung-Eun; Choi, Young-Sook; Shim, In-Kyong; Lee, Seung-Jin; Seol, Yang-Jo; Chung, Chong-Pyoung; Park, Yoon-Jeong
Issue Date
2009
Publisher
Portland Press
Citation
Biotechnol. Appl. Biochem. 52, 69–77
Keywords
bone regenerative therapycell-binding domainchitosan membranefibroblast growth factor-2mesenchymal stem cellosteoblastic differentiation
Abstract
Two cell-binding domains from FGF-2 (fibroblast growth factor-2) were shown to increase cell attachment and osteoblastic differentiation. Two synthetic peptides derived from FGF-2, namely residues 36-41 (F36; PDGRVD) and 77-83 (F77; KEDGRLL), were prepared and their N-termini further modified for ease of surface immobilization. Chitosan membranes were used in the present study as mechanical supportive biomaterials for peptide immobilization. Peptides could be stably immobilized on to the surface of chitosan membranes. The adhesion of mesenchymal stem cells to the peptide (F36 and F77)-immobilized chitosan membrane was increased in a dose-dependent manner and completely inhibited by soluble RGD (Arg-Gly-Asp) and anti-integrin antibody, indicating the existence of an interaction between F36/F77 and integrin. Peptide-immobilized chitosan supported human bone-marrow-derived mesenchymal-stem-cell differentiation into osteoblastic cells, as demonstrated by alkaline phosphate expression and mineralization. Taken together, the identified peptide-immobilized chitosan membranes were able to support cell adhesion and osteoblastic differentiation; thus these peptides might be useful as bioactive agents for osteoblastic differentiation and surface-modification tools in bone regenerative therapy.
ISSN
0885-4513
Language
English
URI
http://hdl.handle.net/10371/68696
DOI
https://doi.org/10.1042/BA20070169
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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