Influences of Carbachol on Potassium Channels in Sublingual Acinar Cells of Rat

Abstract

The Salive is formed by outflow of potassium ion after calcium ion influx into acinar cell which was activated by secretagogue. The channel related withe the outflow of potassium ion channel is one of the channels first studies with patch clamp technique in epithelial cell. Contrary to the many studies of submandibular gland, there has been no work about the electrophysiological study of sublingual gland with patch clamp technique. The sublingual acinar cells were collected from Spraue-Dawley ray by time-scheduled trypsin and solution with 2-5 MΩ resistance were used. Membrane currents were recorded by cell attached patch clamp method. 10㎛ Acetylcholine(ACh) or 10㎛ Carbachol(CCh) was directly added in perfusion solution. The channel activities were recorded and were evaluated with personal computer on pClamp software. The obtained results were as followed. 1. We could attain the isolated single acinar cells of sublingual gland. 2. The conductance of potassium ion channel was about 190pS and voltage dependent. 3. The other type of channel was not sensitive to pipette voltage and had small conductance. 4. Addition of ACh or CCh increased the open probability of potassium channels without change of conductance.