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Transforming growth factor (TGF)-beta1 releasing tricalcium phosphate/chitosan microgranules as bone substitutes

Cited 23 time in Web of Science Cited 22 time in Scopus
Authors
Lee, Jue-Yeon; Seol, Yang-Jo; Kim, Kyoung-Hwa; Lee, Yong-Moo; Park, Yoon-Jeong; Rhyu, In-Chul; Chung, Chong-Pyoung; Lee, Seung-Jin
Issue Date
2004-10
Publisher
Springer Verlag
Citation
Pharmaceutical Research 21:1790-1796
Abstract
Purpose. Tricalcium phosphate (TCP)/chitosan composite microgranules were developed as bone substitutes and tissue engineering scaffolds with the aim of obtaining a high bone forming efficacy. The microgranules have the ability to fill various types of defect sites with closer packing. In addition, the transforming growth factor-beta 1 (TGF-1) was added to the microgranules in order to improve bone-healing efficacy.
Methods. TCP/chitosan microgranules were fabricated by dropping a TCP suspended chitosan solution into a NaOH/ethanol solution. TGF-1 was incorporated into the TCP/chitosan microgranules by soaking the microgranules into the TGF-1 solution. Scanning electron microscopy (SEM) observations as well as experiments examining the release of TGF-1 from chitosan and TCP/chitosan microgranules were performed. SEM was used to examine the cell morphologies on the microgranules, and the extent of cell proliferation was evaluated using a dimethyl-thiazol tetrazolium bromide (MTT) assay. The differentiated cell function was assessed by measuring the alkaline phosphatase activity as well as performing an osteocalcin assay.
Results. The size of the prepared microgranules was 350-500m and TCP powders were observed on the surface of the microgranules. TGF-1 was released from the TCP/chitosan microgranules at a therapeutic concentration for 4 weeks. The proliferation of osteoblasts on the TGF-1 loaded microgranules was the highest among the microgranules. SEM indicated that the seeded osteoblastic cells were firmly attached to the microgranules and proliferated in a multilayer fashion. The ALPase activity and osteocalcin content of all the samples increased during the culture period.
Conclusions. These results suggest that the TCP/chitosan microgranules are potential bone substitutes with a drug releasing capacity and a osteoblastic cells culture scaffold.
ISSN
0724-8741
Language
English
URI
http://hdl.handle.net/10371/68905
DOI
https://doi.org/10.1023/B:PHAM.0000045230.14287.73
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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