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Effect of Smear Layer and Chlorhexidine Treatment on the Adhesion of Enterococcus faecalis to Bovine Dentin

Cited 19 time in Web of Science Cited 17 time in Scopus
Authors
Yang, Seung-Eun; Cha, Jeong-Heon; Kim, Eui-Seong; Kum, Kee-Yeon; Lee, Chang-Young; Jung, Il-Young
Issue Date
2006-07
Publisher
Elsevier
Citation
J Endod 2006;32:663– 667
Keywords
AdhesionchlorhexidineEnterococcus faecalissmear layer
Abstract
The aim of this in vitro study was to determine the
effects of a smear layer and chlorhexidine (CHX) treatment
on the adhesion of Enterococcus faecalis to bovine
dentin. Forty dentin blocks from bovine incisors
were prepared and randomly divided into four groups
of 10 each. The blocks in group 1 were placed in sterile
saline for 5 minutes, while those in group 2 were
treated with 17% EDTA for 5 minutes. The blocks in
group 3 were placed in 2% CHX for 7 days. The blocks
in group 4 were treated with 17% EDTA for 5 minutes,
and then placed in 2% CHX for 7 days. All the blocks
were immersed in a suspension of E. faecalis for 3
hours. The bacteria adhering to the dentin surface were
counted by examination using a scanning electron microscope.
The most significant amount of bacteria was
retained on the samples from group 1 (p 0.05) and
the smallest amount of bacteria adhered to the samples
from group 4. These results suggest that a smear layer
enhances the adherence of E. faecalis to the dentin, and
CHX is effective in reducing the adherence of
microorganisms.
ISSN
0099-2399
Language
English
URI
http://hdl.handle.net/10371/68958
DOI
https://doi.org/10.1016/j.joen.2005.12.002
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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