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Effects of Protein Kinase C Modulators on the Osteoclast-like Cell Generation Induced by Osteotropic Hormones

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Authors
Ko, Seong-Hee; Lee, In-Seok; Kim, Gwan-Shik
Issue Date
1995
Publisher
Korean Academy of Oral Biology
Citation
Int. J. of Oral Biol 19(1):17-54
Keywords
protein kinase CPMAH7osteoclast-like cellsosteotropic hormones
Abstract
Protein kinase C (PKC) has been known to play an important role in the process of cell differentiation. The present study was undertaken to investigate the effect of modulators of PKC in the generation of osteoclast-like cells from mouse bone marrow cells. The marrow cells were collected from the femurs and tibiae of 4- to 6-week-old mice and cultured for 8 days. In order to examine the role of PKC in the generation of osteoclast-like cells induced by hormones, a PKC modulator, PMA or H7 was supplemented in the presence of PGE_2, 1,25-dihydroxyvitamin D_3, or PTH. After culture, the cells were stained for tartrate-resistant acid phosphatase (TRACP), a marker enzyme of osteoclast and the TRACP-positive multinucleated cells (MNCs) which have 3 or more nuclei were counted.
The present study showed that TRACP-positive MNCs were not formed in the presence of PMA and H7 alone. However, the generation of TRACP-positive MNCs induced by PGE_2 and 1,25-dihydroxyvitamin D_3 was stimulated by PMA at 10^-9 - 10^-7 M. This augmentation of TRACP-positive MNC generation was inhibited by the presence of 10^-5 M H7. The generation of TRACP-positive MNCs induced by PTH was suppressed by 10^-9 - 10^-7 M PMA. This suppression of TRACP-positive MNC generation by 10^-7 M PMA was completely reversed by 10^-5 M H7. H7 at 10^-5 M inhibited PGE_2 induced TRACP-positive MNC generation. These results suggest that PKC may play an important role in the generation of osteoclasts and may modulate the action of osteotropic hormones in the bone resorption.
ISSN
1226-7155
Language
English
URI
http://hdl.handle.net/10371/68967
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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