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Effect of magnetic field on the fibronectin adsorption, cell attachment and proliferation on titanium surface

Cited 19 time in Web of Science Cited 21 time in Scopus
Authors
Kim, Hyo Jin; Chang, Ik Tae; Heo, Seong Joo; Koak, Jai Young; Kim, Seong Kyun; Jang, Jun Hyeog
Issue Date
2005-10
Publisher
Wiley-Blackwell
Citation
Clin Oral Implants Res. 16(5):557-562
Keywords
cell attachmentcell proliferationfibronectinmagnetic fieldtitanium
Abstract
Objectives: We studied the effect of various static magnetic fields (SMFs) on the adsorption of specific recombinant fibronectin (FN) peptide (hFNIII9-10) on the titanium surface. Furthermore, the responses of human osteosarcoma TE-85 cells in the SMF were observed.

Material and methods: Various magnetic fields – 1, 2, 3, 5, 7, 10 mT – were established by controlling the distance from Nd–Fe–B magnet to the disks. For FN adsorption experiment, machined titanium disks were incubated in 1 μM hFNIII9-10 at 37°C overnight under magnetic field. The adsorbed hFNIII9-10 was measured as optical density (OD). For attachment study, TE-85 cells were incubated for 2 h on the hFNIII9-10 coated machined titanium disks and OD values were measured. As for proliferation study, titanium disks were incubated for 48 h after washing unattached cells in 2 h. The amount of proliferated TE-85 cell was also measured as OD value. Attachments of TE-85 cells under various intensities of magnetic field were observed using a scanning electron microscope.

Results: The amount of adsorbed hFNIII9-10 showed no significant difference between control (0 mT) and six experimental groups (1, 2, 3, 5, 7, 10 mT). However, TE-85 cells attached significantly higher in groups of 1, 2, 5, 10 mT than in control group (P=0). Cell attachment in groups of 3, 7 mT showed no significant difference with that of control group. TE-85 cells were observed to attach through filopodia. Especially in 1 mT, flattened cells were predominant. In proliferation assay, 1 mT stimulated TE-85 cells showed significantly higher proliferation than those in 2, 3 and 7 mT (P=0).

Conclusion: Magnetic fields under 10 mT did not influence FN adsorption on the titanium surface. However, a significant effect was found on cell attachment and proliferation.
ISSN
0905-7161
Language
English
URI
http://hdl.handle.net/10371/68968
DOI
https://doi.org/10.1111/j.1600-0501.2005.01164.x
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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