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Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo

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Authors
Kim, You-Kyoung; Park, In-Kyu; Jiang, Hu-Lin; Choi, Jae-Young; Jea, Yeon-Ho; Jin, Hua; Kim, Hyun-Woo; Cho, Myung-Haing; Cho, Chong-Su
Issue Date
2006-04-03
Publisher
Elsevier
Citation
J. Biotechol. 125, 104–109
Keywords
PegylationBaculovirusGreen fluorescent protein (GFP)Transduction efficiency
Abstract
In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in
vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay.
The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the
transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in
vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with
baculovirus itself possibly owing to increased stability of baculovirus by pegylation.
ISSN
0168-1656
Language
English
URI
http://hdl.handle.net/10371/6898
DOI
https://doi.org/10.1016/j.jbiotec.2006.02.015
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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