S-Space College of Veterinary Medicine (수의과대학) Dept. of Veterinary Medicine (수의학과) Journal Papers (저널논문_수의학과)
Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo
|dc.identifier.citation||J. Biotechol. 125, 104–109||en|
|dc.description.abstract||In this study, poly(ethylene glycol) (PEG) was coupled to baculovirus to regulate transduction efficiency of baculovirus in
vitro and in vivo. The degree of pegylation in virions was measured by the loss of free amines via a fluorescamine-based assay.
The efficiency of green fluorescent protein (GFP) expression was used to monitor transduction efficiency. As the results, the
transduction efficiency in pegylated baculovirus was decreased with an increase of pegylation in baculovirus in vitro and in
vivo. Interestingly, the transduction efficiency of the pegylated baculovirus for the lung and brain was increased compared with
baculovirus itself possibly owing to increased stability of baculovirus by pegylation.
|dc.description.sponsorship||This work was supported by grants from Ministry
of Science and Technology in Korea (M10414030002-
05N1403-00210). We are grateful to the National
Instrument Center for Environmental management
(NICEM) for giving chance of measurement of fluorescence
spectroscopy. Y.K. Kim was supported by Brain
Korea 21 Program.
|dc.subject||Green fluorescent protein (GFP)||en|
|dc.title||Regulation of transduction efficiency by pegylation of baculovirus vector in vitro and in vivo||en|
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