Reversal of the TPA-induced inhibition of gap junctional intercellular communication by Chaga mushroom (Inonotus obliquus) extracts: Effects on MAP kinases

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Park, Jung-Ran; Park, Joon-Suk; Jo, Eun-Hye; Hwang, Jae-Woong; Kim, Sun-Jung; Ra, Jeong-Chan; Aruoma, Okezie I.; Lee, Yong Soon; Kang, Kyung-Sun
Issue Date
IOS Press
BioFactors 27 (2006), 147-155
Inonotus obliquus (Chaga mushroom)gap junctional intercellular communicationconnexin 43ERK 1/2p38 MAP kinasechemoprevention and signaling mechanisms
Chaga mushroom (Inonotus obliquus) has continued to receive attention as a folk medicine with indications for the
treatment of cancers and digestive diseases. The anticarcinogenic effect of Chaga mushroom extract was investigated using a
model system of gap junctional intercellular communication (GJIC) in WB-F344 normal rat liver epithelial cells. The cells
were pre-incubated with Chaga mushroom extracts (5, 10, 20 μg/ml) for 24 h and this was followed by co-treatment with Chaga
mushroom extracts and TPA (12-O-tetradecanoylphorbol-13-acetate, 10 ng/ml) for 1 h. The inhibition of GJIC by TPA (12-Otetradecanoylphorbol-
13-acetate), promoter of cancer, was prevented with treatment of Chaga mushroom extracts. Similarly,
the increased phosphorylated ERK1/2 and p38 protein kinases were markedly reduced in Chaga mushroom extracts-treated
cells. There was no change in the JNK kinase protein level, suggesting that Chaga mushroom extracts could only block the
activation of ERK1/2 and p38 MAP kinase. The Chaga mushroom extracts further prevented the inhibition of GJIC through the
blocking of Cx43 phosphorylation. Indeed cell-to-cell communication through gap junctional channels is a critical factor in the
life and death balance of cells because GJIC has an important function in maintaining tissue homeostasis through the regulation
of cell growth, differentiation, apoptosis and adaptive functions of differentiated cells. Thus Chaga mushroom may act as a
natural anticancer product by preventing the inhibition of GJIC through the inactivation of ERK1/2 and p38 MAP kinase.
0951-6433 (print)
1872-8081 (online)
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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