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Regulation of ADAMTS-2 by 1,25-dihydroxyvitaminD3 in osteoblastic cells.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Seungbok | - |
dc.contributor.author | Jeon, Eun-Young | - |
dc.contributor.author | Kim, Hyun-Man | - |
dc.date.accessioned | 2010-09-02T07:14:21Z | - |
dc.date.available | 2010-09-02T07:14:21Z | - |
dc.date.issued | 2006-08 | - |
dc.identifier.citation | International Journal of Oral Biology. 31:93-98 | en |
dc.identifier.issn | 1226-7155 | - |
dc.identifier.uri | https://hdl.handle.net/10371/69587 | - |
dc.description.abstract | Biosynthetic processing of fibrillar procollagens is
essential for producing mature collagen monomers that polymerize into fibrils by a self-assembly process. The metalloproteinase ADAMTS-2 is the major enzyme that processes the N-propeptide of type I procollagen in the skin and also of type II and type III procollagens. Mutations in the ADAMTS-2 gene cause dermatospraxis in animals and Ehlers-Danlos syndrome VIIC in humans, both of which are characterized by the accumulation of type I pN-collagen and the formation of abnormal collagen fibrils in the skin. Despite its importance in procollagen processing, little is known about the regulation of ADAMTS-2 expression. Here, we demonstrate that ADAMTS-2 can be regulated by 1,25-dihydroxyvitamin D3, an inducer of type I procollagen synthesis. This steroid hormone induced ADAMTS-2 mRNA ~3-fold in MG-63 human osteosarcoma cells and MC3T3-E1 murine osteoblastic cells. This induction was dose- and time-dependent in MG-63 cells. In contrast, secreted ADAMTS-2 protein was increased only 1.4-fold with 1,25-dihydroxyvitamin D3. Finally, 1,25-dihydroxyvitamin D3 in the presence of ascorbate increased levels of secreted ADAMTS-2 1.9-fold over ascorbate treatment alone, which did not appreciably change ADAMTS-2 expression. These data indicate that the regulation of ADAMTS-2 is coupled with the synthesis of type I procollagen through 1,25- dihydroxyvitamin D3 signaling and may involve translational or posttranslational control. | en |
dc.language.iso | en | en |
dc.publisher | Korean Acadamy of Oral Biology | en |
dc.title | Regulation of ADAMTS-2 by 1,25-dihydroxyvitaminD3 in osteoblastic cells. | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 이승복 | - |
dc.contributor.AlternativeAuthor | 전윤영 | - |
dc.contributor.AlternativeAuthor | 김현만 | - |
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