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Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

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Authors
Cho, Eun Seong; Lee, So Yeong; Park, Jae-Yong; Hong, Seong-Geun; Ryu, Pan Dong
Issue Date
2007
Publisher
대한수의학회 = The Korean Society of Veterinary Science
Citation
J Vet Sci 2007, 8, 15-20
Keywords
electrophysiologygreen fluorescent proteingreen fluorescent proteinimmunocytochemistryorganotypic slice cultureparaventricular nucleus
Abstract
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and corticotropin-releasing hormone, and the electrophysiological properties of PVN neurons for up to 3 weeks in vitro. The PVN neurons in the culture expressed the green fluorescent protein gene that had been delivered by the adenoviral vectors. The results indicate that the cultured slices preserve the properties of the PVN neurons, and can be used in longterm studies on these neurons in vitro.
ISSN
1229-845X
Language
English
URI
http://www.vetsci.org/2007/abstract/15a.html

http://hdl.handle.net/10371/6966
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College of Veterinary Medicine (수의과대학)Dept. of Veterinary Medicine (수의학과)Journal Papers (저널논문_수의학과)
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