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Structure and in vitro cytocompatibility of the gastropod shell of Helix pomatia

Cited 4 time in Web of Science Cited 5 time in Scopus
Authors
Han, J.S; Bächle, M; Hübner, U; Kohal, R.J; Wiedmann-Al-Ahmad, M
Issue Date
2006-10
Publisher
Elsevier
Citation
Tissue and Cell 38,337-344
Keywords
Gastropod shellXRDSEMOsteoblast-like cellsCell proliferationCell viability
Abstract
Distinguishing features of biological constructions are high stability and adaptation to their environment. Beside biocompatibility, nontoxicity and degradability these characteristics are demanded for new biomaterials in the field of tissue engineering. This study investigated the chemical composition, the organization and the in vitro osteoconductive potential of the terrestrial gastropod shell (Helix pomatia) on CAL72 and human osteoblast-like cells. Chemical composition of the biomaterial was examined by X-ray diffraction (XRD) and scanning electron microscopy (SEM) was performed to analyze the architecture of the snail shell and the morphology of the seeded cells. A double staining procedure (FDA/PI) and a proliferation test (EZ4U) assessed the viability of the cells. Microscopical images showed the multilayered architecture of the aragonite shell with hexagonal crystals on the inner side. The cells spread well on the biomaterial and the highest proliferation rate could be measured with CAL72 cells on the inner shell surface. The osteoconductive effects of this natural biomaterial could encourage further experiments in the field of tissue engineering.
ISSN
0040-8166
Language
English
URI
http://hdl.handle.net/10371/69675
DOI
https://doi.org/10.1016/j.tice.2006.08.004
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College of Dentistry/School of Dentistry (치과대학/치의학대학원)Dept. of Dentistry (치의학과)Journal Papers (저널논문_치의학과)
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