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Identification of cell surface receptors for murine macrophage inflammatory protein-1alpha
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Kack-Kyun | - |
dc.contributor.author | Oh, Kwi-Ok | - |
dc.contributor.author | Zhou, Zhen | - |
dc.contributor.author | Samanta, Himadri | - |
dc.contributor.author | Fraser, Malcolm | - |
dc.contributor.author | Kim, Young-June | - |
dc.contributor.author | Broxmeyer, Hal E. | - |
dc.contributor.author | Kwon, Byoung S. | - |
dc.date.accessioned | 2010-09-05T23:42:12Z | - |
dc.date.available | 2010-09-05T23:42:12Z | - |
dc.date.issued | 1991 | - |
dc.identifier.citation | Journal of Immunology 147:2978-2983 | en |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://hdl.handle.net/10371/69700 | - |
dc.description.abstract | We have produced recombinant proteins for a cytokine, L2G25BP (macrophage inflammatory protein-1 alpha) (MIP-1 alpha). By using the recombinant protein (rMIP-1 alpha), receptors for MIP-1 alpha were identified on Con A-stimulated and unstimulated CTLL-R8, a T cell line, and LPS-stimulated RAW 264.7, a macrophage cell line. The 125I-rMIP-1 alpha binds to the receptor in a specific and saturable manner. Scatchard analysis indicated a single class of high affinity receptor, with a Kd of approximately 1.5 x 10(-9) M and approximately 1200 binding sites/Con A-stimulated CTLL-R8 cell and a Kd of 0.9 x 10(-9) M and approximately 380 binding sites/RAW 264.7 cell. 125I-rMIP-1 alpha binding was inhibited by unlabeled rMIP-1 alpha in a dose-dependent manner, but not by IL-1 alpha or IL-2. rMIP-1 alpha inhibited the proliferation of unstimulated CTLL-R8 cells. Rabbit anti-rMIP-1 alpha antibodies blocked the growth-inhibitory effect of the rMIP-1 alpha on CTLL-R8 cells. | en |
dc.language.iso | en | en |
dc.publisher | American Association of Immunologists | en |
dc.title | Identification of cell surface receptors for murine macrophage inflammatory protein-1alpha | en |
dc.type | Article | en |
dc.contributor.AlternativeAuthor | 김각균 | - |
dc.contributor.AlternativeAuthor | 오귀옥 | - |
dc.contributor.AlternativeAuthor | 김영준 | - |
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